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Immunology and Infection

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Bioluminescence Imaging of NADPH Oxidase Activity in Different Animal Models

Article DOI: 10.3791/3925 • 10:42 min • October 22nd, 2012
October 22nd, 2012 •
1, 1, 2,3, 1
1Department of Medicine, Vanderbilt University School of Medicine, 2Departments of Medicine and Immunology, Roswell Park Cancer Institute, 3Department of Medicine, University at Buffalo School of Medicine

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Summary
Automatic Translation

NADPH oxidase is the major source of reactive oxygen species (ROS) in phagocytes. Because of the ephemeral nature of ROS, it is difficult to measure and monitor ROS levels in living animals. A minimally invasive method for serial quantification of ROS in living mice is described.

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Bioluminescence Imaging NADPH Oxidase Activity Animal Models Antibacterial Defense Antifungal Defense Inflammatory Response Real-time Kinetics ROS Generation Research Tool Host Defense Inflammation Injury Chronic Granulomatous Disease CGD Inherited Disorder NADPH Oxidase Components Gp91phox-deficient Mice P47phox-deficient Mice Impaired Host Defense Phagocytes Isoforms Of NADPH Oxidase Quantify ROS Production Models Of Inflammation And Injury
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