Co-culture Migration
Co-culture Migration - Related Videos
08:39
Human T Lymphocyte Isolation, Culture and Analysis of Migration In Vitro
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Cited by 27 •
2010
T lymphocyte migration occurs during homing to lymphoid organs, exit from the vasculature, and entering into peripheral tissues. Here, we describe a protocol that can be used to analyze T lymphocyte migration in vitro.
11:20
Quantitative Assessment of Human Neutrophil Migration Across a Cultured Bladder Epithelium
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Cited by 8 •
2013
We developed an in vitro model that mimics an important component of the acute inflammatory response during infection of the bladder with uropathogenic Escherichia coli. The transuroepithelial neutrophil migration assay enables quantitative assessment of human neutrophil migration across bladder epithelia, cultured on permeable supports, in response to bacterial infection or chemoattractant substances.
10:57
Mouse Hindbrain Ex Vivo Culture to Study Facial Branchiomotor Neuron Migration
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Cited by 4 •
2014
Embryonic neurons are born in the ventricular zone of the neural tube, but migrate to reach appropriate targets. Facial branchiomotor (FBM) neurons are a useful model to study neuronal migration. This protocol describes the wholemount ex vivo culture of mouse embryo hindbrains to investigate mechanisms that regulate FBM migration.
08:29
Ex vivo Culture of Mouse Embryonic Skin and Live-imaging of Melanoblast Migration
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Cited by 11 •
2014
We describe the dissection and ex vivo culture of mouse embryonic skin. The culture system maintains an air-liquid interface across the tissue surface and allows imaging on an inverted microscope. Melanoblasts, a component of the developing skin, are fluorescently labeled allowing their behavior to be observed using confocal microscopy.
07:44
Epicardial Outgrowth Culture Assay and Ex Vivo Assessment of Epicardial-derived Cell Migration
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Cited by 9 •
2016
Here, we describe methods for isolating primary mouse epicardial cells by an outgrowth culture assay and assessing the functional migration of epicardial-derived cells (EPDC) using an ex vivo heart culture system. These protocols are suitable for identifying genetic and chemical modulators of epicardial epithelial-to-mesenchymal transition (EMT) and motility.
FAQs
Chemokines and cytokines released by infected, stimulated, or barrier-forming cells can establish concentration gradients within the culture model. Leukocytes respond to these spatially distributed signals, which helps determine the direction and extent of movement. Measuring this response can reveal how host or pathogen-associated stimulation converts cellular signaling into immune-cell recruitment.
Migration depends not only on soluble signaling but also on changes in adhesion between cells and the culture surface or membrane. Signals from one population may alter how readily leukocytes attach, spread, or cross a model boundary. Consequently, differences in measured movement may reflect both guidance by chemokines or cytokines and altered adhesive interactions.
Transwell assays measure movement across a membrane, whereas layered cultures model interactions within an arranged cellular structure. Live-cell imaging adds temporal information by recording movement as it occurs. Selecting among these formats depends on whether the experiment emphasizes migration across a barrier, spatial cell interactions, or dynamic changes in recruitment.
The model pairs a signaling population with a responding population. The first may consist of infected, stimulated, or barrier-forming host cells, while the second may contain leukocytes whose recruitment is measured. Comparing migration under these different co-culture conditions helps connect the state of host cells with the behavior of immune cells.
Researchers can apply the approach when they need to examine how infected cells influence immune-cell recruitment or how pathogen-associated signaling changes cellular interactions. The resulting measurements support analysis of inflammatory responses and pathogen-driven immune signaling. This makes the method relevant for studying communication between host cells and leukocytes within a shared experimental model.
Migration measurements can show whether a candidate treatment changes recruitment or movement within the co-culture system. Reduced or altered migration may indicate an effect on inflammatory signaling, host-cell communication, or interactions associated with infection. These results help investigators evaluate treatment effects while linking cellular movement to broader immune and pathogen-related responses.