Cytokine Release Assay
Cytokine Release Assay - Related Videos
06:37
Using Reference Reagents to Confirm Robustness of Cytokine Release Assays for the Prediction of Monoclonal Antibody Safety
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2023
The use of cytokine release assay reference reagents allows for more reproducible and standardized in vitro safety profiles of immunotherapeutic monoclonal antibodies. Here we describe how cytokine release assays can be used alongside a reference reagent panel to predict the safety of some therapeutic monoclonal antibodies.
09:22
Bead Based Multiplex Assay for Analysis of Tear Cytokine Profiles
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Cited by 12 •
2017
Analysis of tear film cytokines helps in studying various ocular diseases. Bead based multiplex assays are simple and sensitive and enable the testing of multiple targets in samples with small volumes. Here we describe a protocol for tear film cytokine profiling a using bead based multiplex assay.
06:29
Accurate and Simple Measurement of the Pro-inflammatory Cytokine IL-1β using a Whole Blood Stimulation Assay
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Cited by 9 •
2011
We describe a simple immunoassay to measure the production of pro-inflammatory cytokines, such as IL-1 beta production, in patients presenting with autoinflammatory phenotypes. By activating cells in whole blood cultures with pathogen-associated molecular patterns, specifically with lipopolysaccharide, cytokine secretion can be conveniently evaluated in whole blood supernatants.
04:46
A Multiplex Bead-Based Assay for Analyzing Cytokines in Human Tear Samples
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2025
This video illustrates a bead-based multiplex assay for analyzing cytokines in human tears. The method allows for the simultaneous analysis of multiple cytokines from a single sample, providing enhanced sensitivity for studying immune responses in various pathophysiological conditions.
03:02
A Lactate Dehydrogenase Release Assay To Detect Macrophage Death Following Nigericin Exposure
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2025
This video demonstrates the lactate dehydrogenase assay to detect inflammasome-mediated macrophage death. The macrophages are exposed to lipopolysaccharide for priming, followed by exposure to nigericin to cause inflammasome-induced cell death, which is determined by the lactate dehydrogenase assay.
FAQs
The stimulus determines which immune response is being examined. Antigens can probe antigen-specific immunity, pathogen-associated components can model responses linked to infection, and therapeutic compounds can reveal immune activation associated with treatment exposure. Comparing cytokine secretion after different stimuli helps distinguish stimulus-linked cellular activation from broader inflammatory behavior.
A set of cytokines can show different features of the response at the same time. Interferons, interleukins, and tumor necrosis factor may contribute to distinct patterns of cellular activation and inflammation. Examining their combined secretion provides a broader profile of immune regulation than relying on one measured signal alone.
Both approaches quantify cytokines released by stimulated immune cells, but they differ in measurement format. An immunoassay can assess selected cytokine signals, whereas a multiplex detection platform measures multiple cytokines within the same analytical workflow. Multiplex results can therefore support broader characterization of inflammatory patterns when several cytokines are relevant.
A typical workflow begins with blood-derived or cultured immune cells, followed by exposure to a chosen antigen, pathogen-associated component, or therapeutic compound. After the cells respond, the released cytokines are collected for quantification using an immunoassay or multiplex detection platform. The resulting measurements are then interpreted as evidence of cellular activation and inflammatory response.
The assay can use immune cells derived from blood or maintained in culture, depending on the experimental question. Researchers may expose them to an antigen, a pathogen-associated component, or a therapeutic compound. This flexibility allows the same general approach to examine antigen-specific immunity, infection-related responses, or treatment-associated immune activation.
In immunology and infection research, the measurements can help characterize host responses to infection and evaluate antigen-specific immunity. The approach also supports investigation of vaccine responses by showing cytokine patterns after relevant stimulation. In therapeutic studies, it can identify drug-related immune activation and provide evidence of associated inflammatory behavior.