Ex Vivo Lung Imaging
Ex Vivo Lung Imaging - Related Videos
11:19
Ex vivo Live Imaging of Lung Metastasis and Their Microenvironment
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Cited by 6 •
2016
We describe a relatively simple method for ex vivo live imaging of the tumor cell-stroma interactions within lung metastasis, utilizing fluorescent reporters in mice. Using spinning-disk confocal microscopy, this technique enables visualization of live cells for at least 4 hr and could be adapted to study other inflammatory lung conditions.
05:55
Whole Lung Agarose Inflation: A Method of Preparing Lungs for Ex vivo Live Imaging
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2023
This video describes a method of preparing lungs for ex vivo live imaging to study interactions between cancer cells and stromal cells, using agarose for inflation. In the sample protocol, agarose inflation procedure is described before observing live cellular interactions.
05:49
Establishment of an Ex Vivo Lung Perfusion Rat Model for Translational Insights in Lung Transplantation
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Cited by 5 •
2023
Here, we describe the necessary steps for establishing a rat EVLP model and show the inflammatory profile associated with the perfused lungs. The aim is to propagate knowledge and experiences about the rat EVLP model, enabling the integral understanding of the biological responses associated with that revolutionary technique.
08:59
Method of Isolated Ex Vivo Lung Perfusion in a Rat Model: Lessons Learned from Developing a Rat EVLP Program
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Cited by 27 •
2015
Ex-Vivo Lung Perfusion (EVLP) has allowed lung transplantation in humans to become more readily available by enabling the ability to assess organs and expand the donor pool. Here, we describe the development of a rat EVLP program and refinements that allow for a reproducible model for future expansion.
08:28
Ex Vivo Imaging of Resident CD8 T Lymphocytes in Human Lung Tumor Slices Using Confocal Microscopy
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Cited by 21 •
2017
This protocol describes a method to image resident tumor-infiltrating CD8 T cells labeled with fluorescently coupled antibodies within human lung tumor slices. This technique permits real-time analyses of CD8 T cell migration using confocal microscopy.
FAQs
Preserved lung architecture allows investigators to examine immune and microbial behavior in relation to epithelial and vascular compartments rather than as isolated cells. Maintaining the tissue under defined conditions also helps connect observed cell migration or pathogen localization with changes occurring across the pulmonary environment. This context is especially valuable for interpreting complex infection and inflammation responses.
Ventilation and perfusion provide controlled ways to maintain relevant lung conditions during imaging. They can help investigators examine how cellular responses and pathogen interactions unfold while tissue remains supported outside the body. Fluorescent labels and time-lapse microscopy add temporal and visual information, allowing movement and behavior to be followed rather than inferred only from endpoint measurements.
Fluorescent labeling identifies selected cells or microorganisms, while time-lapse microscopy records their behavior over time. Together, these tools can show where immune cells migrate, how pathogens associate with lung compartments, and when inflammation-related changes emerge. The resulting images link cellular activity to preserved tissue structure and support interpretation of dynamic infection or immune responses.
A typical workflow begins with removal of lung tissue or a whole lung, followed by maintenance under defined laboratory conditions. Depending on the study, investigators incorporate ventilation, perfusion, fluorescent labeling, or time-lapse microscopy. Imaging then captures responses within the maintained preparation, enabling analysis of structure, immune activity, or pathogen behavior in preserved pulmonary tissue.
In infection studies, imaging can examine interactions separately across epithelial and vascular compartments rather than treating the lung as a uniform sample. It may show where pathogens encounter tissue barriers, where immune cells move, and how inflammation changes observable lung function. These spatially resolved observations provide context for interpreting host responses within the maintained lung preparation.
Ex vivo lung imaging can complement animal models and cell-culture systems by preserving tissue complexity while allowing more controlled observation. This makes it relevant for evaluating host-directed therapies alongside antimicrobial strategies. Findings can help assess treatment-associated changes in immune activity, pathogen behavior, or inflammation within the lung preparation, adding tissue-level context to therapeutic studies.