Fluorescent Muscle Transplantation
Fluorescent Muscle Transplantation - Related Videos
09:39
Normal and Malignant Muscle Cell Transplantation into Immune Compromised Adult Zebrafish
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Cited by 16 •
2014
Here, we present a protocol for cell transplantation of zebrafish skeletal muscle and embryonal rhabdomyosarcoma (ERMS) into adult immune compromised rag2E450fs homozygous mutant zebrafish. This protocol allows for the efficient analysis of regeneration and malignant transformation of muscle cells.
09:24
Molecular Imaging to Target Transplanted Muscle Progenitor Cells
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Cited by 3 •
2013
A non-invasive means to evaluate the success of myoblast transplantation is described. The method takes advantage of a unified fusion reporter gene composed of genes whose expression can be imaged with different imaging modalities. Here, we make use of a fluc reporter gene sequence to target cells via bioluminescence imaging.
08:15
Tracking Dynamics of Muscle Engraftment in Small Animals by In Vivo Fluorescent Imaging
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Cited by 4 •
2009
We describe an in vivo fluorescence imaging protocol to monitor muscle regeneration by GFP-labeled myoblasts after transplantation into skeletal muscles of both healthy and dystrophic mice. This protocol can be adapted to study muscle regeneration by transplantation of other types of cells and in other muscular conditions as well.
10:03
Transplantation of Induced Pluripotent Stem Cell-derived Mesoangioblast-like Myogenic Progenitors in Mouse Models of Muscle Regeneration
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Cited by 30 •
2014
Induced pluripotent stem cell (iPSC)-derived myogenic progenitors are promising candidates for cell therapy strategies to treat muscular dystrophies. This protocol describes transplantation and functional measurements required to evaluate the engraftment and differentiation of iPSC-derived mesoangioblasts (a type of muscle progenitors) in mouse models of acute and chronic muscle regeneration.
08:15
Cervical Heterotopic Mouse Heart Transplantation using End-to-side Anastomosis Technique to Study Transplant Immunology
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2026
This protocol presents a simplified, end-to-side suture anastomosis technique for murine cervical heart transplantation, providing a robust and reproducible model for studying transplant immunology.
FAQs
The fluorescent label provides a location-specific signal for the transplanted muscle material, allowing investigators to track where the graft remains or changes over time. Comparing that signal with observations of host immune-cell recruitment and inflammation helps relate graft distribution and persistence to the recipient’s response, rather than examining immune activity without spatial context.
A single observation shows only where labeled muscle is detected at one moment. Repeated measurements can reveal whether the transplanted material persists, changes its spatial distribution, or becomes associated with evolving immune responses. This time-dependent view helps distinguish an early response from later patterns of rejection, inflammation, repair, or altered engraftment.
The method connects the location of transplanted muscle with the presence or behavior of host immune cells. Investigators can examine whether immune-cell recruitment accompanies particular graft regions or changes as the graft persists. In immunology research, this spatial and temporal relationship supports analysis of how host defenses respond to transplanted tissue.
A typical workflow begins by fluorescently labeling muscle tissue or cells, transferring the labeled material into a recipient, and detecting the emitted signal after excitation at an appropriate wavelength. Researchers then follow the graft over time, assessing its distribution and persistence while relating those observations to immune-cell recruitment, inflammation, or tissue repair.
The approach can provide visual information about where the graft is located, how long transplanted material remains detectable, and how its behavior relates to host responses. These observations support assessment of graft rejection, immune-cell recruitment, inflammatory changes, and tissue repair, creating a readout that links cellular behavior with overall transplant outcome.
Infection and inflammation research, the method helps examine whether altered host conditions affect graft behavior and engraftment. Researchers can follow changes in the labeled muscle while evaluating associated immune-cell recruitment and inflammatory responses. This makes it useful for connecting infection-related or inflammatory changes with transplant persistence, rejection, and repair.