Foxp3 Cd25 Cd4 Cd8
Foxp3 Cd25 Cd4 Cd8 - Related Videos
08:20
In Vitro Differentiation of Human CD4+FOXP3+ Induced Regulatory T Cells (iTregs) from Naïve CD4+ T Cells Using a TGF-β-containing Protocol
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Cited by 24 •
2016
This protocol describes the reproducible generation and phenotyping of human induced regulatory T cells (iTregs) from naïve CD4+ T cells in vitro. Different protocols for FOXP3 induction allow for the study of specific iTreg phenotypes obtained with respective protocols.
10:57
Cell Cycle Analysis: Assessing CD4 and CD8 T Cell Proliferation After Stimulation Using CFSE Staining and Flow Cytometry
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2023
Source: Perchet Thibaut1,2,3, Meunier Sylvain1,2,3, Sophie Novault4, Rachel Golub1,2,3 1 Unit for Lymphpoiesis, Department of Immunology, Pasteur Institute, Paris, France 2 INSERM U1223, Paris, France 3 Université Paris Diderot, Sorbonne Paris Cité, Cellule Pasteur, Paris, France 4 Flow Cytometry Platfrom, Cytometry and Biomarkers UtechS, Center for Translational Science, Pasteur Institute, Paris, France The cell cycle is a universal process of life. During the cell cycle, a cell undergoes...
07:17
Phenotypic and Functional Analysis of Activated Regulatory T Cells Isolated from Chronic Lymphocytic Choriomeningitis Virus-infected Mice
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Cited by 1 •
2016
Here, we describe a protocol to analyze the phenotype of regulatory T (Treg) cells isolated from naïve and chronic lymphocytic choriomeningitis virus-infected mice. In addition, we provide a process to evaluate the suppressive activity of the Treg cells.
10:00
Preparation of CD4+ T Cells for Analysis of GD3 and GD2 Ganglioside Membrane Expression by Microscopy
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Cited by 2 •
2016
We describe a standard antibody staining protocol for use in microscopy to determine the membrane expression and localization of gangliosides in resting and activated human naïve CD4+ T cells. Also described are real-time PCR experiments using <40,000 cells that do not require additional low input RNA kits.
06:08
Generating Single Positive CD8 T Cells from Induced Pluripotent Stem Cells
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2025
This video demonstrates an assay to generate tumor antigen-specific induced pluripotent stem cell (iPSC)-derived CD8αβ+ T Cells. Tumor antigen-specific iPSCs are grown on a stromal cell monolayer and differentiated into hematopoietic progenitor cells (HPCs). The HPCs are differentiated into CD4+ CD8+ double-positive T cells using a differentiation medium. These cells are then stimulated to generate single-positive T cells expressing CD8.
FAQs
CD4 establishes the helper-lineage context, while CD25 and Foxp3 provide additional regulatory-T-cell-associated features. CD25 reflects expression of the interleukin-2 receptor α-chain, and Foxp3 indicates the intracellular transcriptional program associated with regulatory T-cell development and function. Considering these markers together enables more specific identification of CD4+CD25+Foxp3+ populations than relying on CD4 alone.
Foxp3 is an intracellular transcription factor, unlike the surface-associated proteins CD4, CD8, and CD25. This distinction matters because marker location contributes to how a cell population is characterized during flow cytometry. Including Foxp3 adds information about regulatory T-cell development and function, complementing surface phenotype measurements that distinguish helper and cytotoxic T-cell lineages.
CD4 and CD8 help separate major T-cell lineages with different immunological associations. CD4 identifies helper-associated populations, whereas CD8 identifies cytotoxic-associated populations. Examining these markers in infection studies allows researchers to characterize changes in helper and cytotoxic T-cell subsets, then relate those patterns to broader immune activation, disease progression, or treatment effects.
Flow cytometry measures the marker pattern on individual cells, allowing researchers to distinguish populations according to combinations of CD4, CD8, CD25, and Foxp3. A typical analysis separates helper-associated and cytotoxic-associated cells through CD4 and CD8, then examines CD25 and intracellular Foxp3 within relevant populations. The resulting profile supports identification of regulatory T cells and characterization of CD8+ subsets.
The panel can show how regulatory, helper-associated, and cytotoxic-associated T-cell populations are represented during an immune response. Researchers can use these profiles to assess immune activation and tolerance while examining patterns associated with disease progression. Because the markers distinguish several T-cell populations, the analysis provides cellular context rather than treating the immune response as a single uniform process.
Combined profiling is useful when a study needs to determine whether an immunomodulatory treatment affects regulatory T-cell-associated populations or broader T-cell composition. Changes in CD4+CD25+Foxp3+ cells can be considered alongside CD8+ subset patterns, helping researchers evaluate treatment-related effects on immune regulation, activation, and tolerance in the context of infection or other immune responses.