Il-13 Expression
Il-13 Expression - Related Videos
01:10
Carbon-13 (¹³C) NMR: Overview
0 Views •
2024
Carbon-13 is a naturally occurring NMR-active isotope of carbon with a low natural abundance of 1.1%. In contrast, carbon-12 is the most abundant isotope of carbon with zero nuclear spin. Therefore, it is NMR inactive. The gyromagnetic ratio of carbon-13 is smaller than that of protons. As a result, carbon-13 resonance is about 6000 times weaker than proton resonance. For a given magnetic field strength, the resonance frequency of carbon-13 is about one-fourth of the resonance frequency for...
![Assessment of Gastric Emptying in Non-obese Diabetic Mice Using a [<sup>13</sup>C]-octanoic Acid Breath Test](/_next/image?url=https%3A%2F%2Fcloudfront.jove.com%2FCDNSource%2Fteasers%2F50301.jpg%3Fv%3D2026-08-29%252018%253A32%253A39&w=750&q=75)
12:24
Assessment of Gastric Emptying in Non-obese Diabetic Mice Using a [13C]-octanoic Acid Breath Test
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Cited by 18 •
2013
Determination of gastric emptying with a non-invasive [13C]-octanoic acid breath test for tracking gastroparesis in female NOD LtJ mice.
07:26
Metabolic Pathway Confirmation and Discovery Through 13C-labeling of Proteinogenic Amino Acids
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Cited by 53 •
2012
13C-isotope labeling is a useful technique for determining the cell central metabolism for various types of microorganisms. After cells have been cultured with a specific labeled substrate, GC-MS measurement can reveal functional metabolic pathways based on unique labeling patterns in proteinogenic amino acids.
13:59
Methods to Identify the NMR Resonances of the 13C-Dimethyl N-terminal Amine on Reductively Methylated Proteins
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Cited by 4 •
2013
Two methods for assigning the α- and ε-dimethylamine nuclear magnetic resonance signals of a reductively 13C-methylated N-terminal lysine are described. One method utilizes the pH-induced selectivity of the reductive methylation reaction, and the other uses aminopeptidase to selectively remove the N-terminal lysine.
![Investigating Cardiac Metabolism in the Isolated Perfused Mouse Heart with Hyperpolarized [1-<sup>13</sup>C]Pyruvate and <sup>13</sup>C/<sup>31</sup>P NMR Spectroscopy](/_next/image?url=https%3A%2F%2Fcloudfront.jove.com%2FCDNSource%2Fteasers%2F63188.jpg%3Fv%3D2026-09-01%252019%253A57%253A02&w=750&q=75)
14:56
Investigating Cardiac Metabolism in the Isolated Perfused Mouse Heart with Hyperpolarized [1-13C]Pyruvate and 13C/31P NMR Spectroscopy
0 Views •
2023
We describe an experimental setup for administrating hyperpolarized 13C-labeled metabolites in continuous perfusion mode to an isolated perfused mouse heart. A dedicated 13C-NMR acquisition approach enabled the quantification of metabolic enzyme activity in real-time, and a multiparametric 31P-NMR analysis enabled the determination of the tissue ATP content and pH.
FAQs
Induction begins when activated T helper 2 cells and innate lymphoid cells engage transcriptional programs that promote IL-13 synthesis. These programs determine when the cytokine is produced and secreted, linking cellular activation to downstream type 2 immune activity. Examining this regulation helps researchers identify which immune populations contribute to a response.
Receptor engagement activates intracellular signaling that includes the JAK-STAT6 pathway. This signaling transmits the extracellular IL-13 stimulus into the responding cell, altering cellular functions associated with type 2 inflammation and tissue responses. Studying this step helps connect measured cytokine production with biological effects rather than treating expression as an isolated event.
The source provides important context for understanding the immune response. Production by activated T helper 2 cells or innate lymphoid cells indicates participation of type 2 immune programs, but the biological interpretation also depends on the surrounding tissue and infection setting. Identifying the contributing population can clarify how host responses are organized.
Measurement can characterize type 2 inflammation and help evaluate host responses during parasitic infection. It can also indicate associations with mucus production and tissue remodeling, providing a broader view of how immune activity affects tissue function. These outcomes make expression analysis useful for linking cytokine regulation to observable disease-related or infection-related responses.
Researchers assess IL-13 expression to characterize the host response associated with parasitic infection, particularly when type 2 immune activity is relevant. The resulting measurements can be considered alongside effects such as tissue remodeling or mucus production. This approach helps describe how immune regulation and tissue function change in an infection context.
IL-13 expression provides a way to examine type 2 inflammation in asthma and allergic disease, while receptor signaling connects those measurements to downstream immune and tissue effects. In therapeutic studies, the same framework supports evaluation of interventions that target IL-13 signaling, helping relate disease-associated biology to potential treatment strategies.