Immuno-rna Fish
Immuno-rna Fish - Related Videos
10:16
Detection of Viral RNA by Fluorescence in situ Hybridization (FISH)
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Cited by 35 •
2012
A fluorescence in situ hybridization (FISH) method was developed to visually detect viral genomic RNA using fluorescence microscopy. A probe is made with specificity to the viral RNA that can then be identified using a combination of hybridization and immunofluorescence techniques. This technique offers the advantage of identifying the localization of the viral RNA or DNA at steady-state, providing information on the control of intracellular virus trafficking events.
02:59
Using Immuno-RNA Fluorescence In Situ Hybridization to Visualize SARS-CoV-2
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2025
This video demonstrates the application of Hybridization Chain Reaction (HCR)-Fluorescence In Situ Hybridization (FISH) for visualizing the spatial distribution of SARS-CoV-2 subgenomic RNA. Combining HCR with immunofluorescence techniques has the potential to provide insights into host-virus interactions at the single-cell level.
13:06
Analysis of Single-cell Gene Transcription by RNA Fluorescent In Situ Hybridization (FISH)
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Cited by 6 •
2012
Fluorescent in situ hybridization (FISH) to identify mRNA transcripts in individual cells allows analysis of polygenic activity such as the simultaneous transcription of more than one member of the var multigene family in Plasmodium falciparum infected erythrocytes 1. The technique is adaptable and can be used on different types of genes, cells and organisms.
07:25
Basic Methods for the Study of Reproductive Ecology of Fish in Aquaria
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Cited by 2 •
2017
A series of basic methods to enable the study of the reproductive ecology of fish kept in aquaria are described. These are useful protocols for collecting fish using SCUBA, transporting live fish, and observing the reproductive behavior of wild-caught fish kept in aquaria.
05:23
Visualization of SARS-CoV-2 using Immuno RNA-Fluorescence In Situ Hybridization
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Cited by 9 •
2020
Here, we describe a simple method that combines RNA fluorescence in situ hybridization (RNA-FISH) with immunofluorescence to visualize severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) RNA. This protocol may increase understanding of the molecular characteristics of SARS-CoV-2 RNA-host interactions at a single-cell level.
FAQs
The method combines two signal-generation steps in the same fixed specimen: fluorescent nucleic acid probes hybridize to selected RNA, while antibodies identify proteins through immunofluorescence. Because both labels are examined by microscopy within the same cellular or tissue material, researchers can compare transcript and protein distributions in their original locations, including possible overlap between signals.
The nucleic acid probes provide sequence-directed detection of target RNA, whereas antibodies supply protein-specific labeling. These signals answer complementary questions: where a transcript is present and where an immune or infection-related protein is located. Using both in one specimen allows spatial comparison of gene-expression signals with immune components rather than examining either molecular class in isolation.
A single-marker approach can show either RNA distribution or protein distribution, but not their relationship in the same spatial map. The combined method links pathogen or host transcripts with immune markers, enabling researchers to assess whether signals occupy the same cellular or tissue regions. This is particularly useful when interpreting localized immune activation or infection sites.
Researchers begin with a fixed cell or tissue specimen, apply fluorescently labeled nucleic acid probes to hybridize with the RNA target, and then perform antibody-based immunofluorescence to label proteins. Microscopy follows both labeling steps and displays the resulting signals together. The workflow is designed to retain spatial context while measuring transcript and protein distributions in the same sample.
The assay requires a fixed cell or tissue context, a fluorescently labeled nucleic acid probe matched to the RNA of interest, and antibodies for protein detection. These components are complementary: fixation provides the specimen framework, probes identify RNA, and immunofluorescence marks proteins. Microscopy then converts the separate fluorescent labels into a spatially interpretable readout.
In infection research, investigators can pair pathogen transcripts with host immune markers or examine host transcripts alongside proteins associated with defense. The resulting spatial patterns help locate infection-related signals and compare them with immune components in cells or tissues. This supports analysis of cellular responses and of how microbial RNA relates spatially to host defense pathways.
The images can show where selected transcripts and proteins accumulate and whether their distributions potentially overlap. In immunology, these patterns help connect gene-expression signals with immune components; in infection studies, they can be compared across relevant cellular or tissue locations. Such spatial evidence supports mechanistic studies of immune activation and the progression of disease-related changes.