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Immunology and Infection

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JoVE Journal Immunology and Infection
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Visualization of SARS-CoV-2 using Immuno RNA-Fluorescence In Situ Hybridization

Article DOI: 10.3791/62067-v • 05:23 min • December 23rd, 2020
December 23rd, 2020 •
1, 2, 1, 1,2, 1,2, 3,4, 3,4, 1
1Malopolska Centre of Biotechnology, Jagiellonian University, 2Microbiology Department Faculty of Biochemistry, Biophysics and Biotechnology, Jagiellonian University, 3Department of Cardiac, Vascular and Endovascular Surgery and Transplantology, The Medical University of Silesia in Katowice, 4Silesian Centre for Heart Diseases

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Summary
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Here, we describe a simple method that combines RNA fluorescence in situ hybridization (RNA-FISH) with immunofluorescence to visualize severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) RNA. This protocol may increase understanding of the molecular characteristics of SARS-CoV-2 RNA-host interactions at a single-cell level.

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SARS-CoV-2 Immuno RNA-Fluorescence In Situ Hybridization Visualize RNA Cell Lines Human Airway Epithelium Cultures Viral Pathogenesis Replication Single-cell Level Specificity Sensitivity Resolution Diagnostics Agnieszka Suder PhD Student Fixing Permeabilizing Cells 1X PBS Solution Amplification Buffer HCR Hairpin Hairpin Solution Incubation Temperature Hairpin Mixture Parafilm Coverslips
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