Ki67-fitc Antibody
Ki67-fitc Antibody - Related Videos
01:58
Studying the Permeation of FITC and FITC-Ferritin through an In Vitro Blood-Brain Barrier Model
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2025
This video demonstrates a method to study permeation through an in vitro blood-brain barrier (BBB) model using astrocytes and endothelial cells on a membrane insert, with free FITC and FITC-loaded ferritin. FITC-ferritin bound to receptors on endothelial cells and crossed the membrane barrier to the lower chamber while free FITC showed restricted permeation and remained in the upper chamber.
02:56
FITC-Dextran Feeding: A Method to Quantify Intestinal Permeability in C. elegans
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2023
This video introduces a method of to visualize and measure the integrity of the C. elegans intestine lumen by feeding worms FITC-labeled dextran. The example protocol shows the assay done with 3,3’-diindolylmethane (DIM)-treated and pathogen-fed worms.
11:13
In Vitro Permeation of FITC-loaded Ferritins Across a Rat Blood-brain Barrier: a Model to Study the Delivery of Nanoformulated Molecules
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Cited by 15 •
2016
A method to establish an in vitro model of blood-brain barrier based on a co-culture of rat brain microvascular endothelial cells and astrocytes is described and validated. This system proved to be a valid tool to study the effect of nanoformulation on the trans-barrier permeation of fluorescent molecules.
04:50
Imaging FITC-dextran as a Reporter for Regulated Exocytosis
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Cited by 10 •
2018
Here we detail a method for live cell imaging of regulated exocytosis. This method utilizes FITC-dextran, which accumulates in lysosome-related organelles, as a reporter. This simple method also allows distinguishing between different modes of regulated exocytosis in cells that are difficult to manipulate genetically.
13:21
Antibody Generation: Producing Monoclonal Antibodies Using Hybridomas
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2023
Source: Frances V. Sjaastad1,2, Whitney Swanson2,3, and Thomas S. Griffith1,2,3,4 1 Microbiology, Immunology, and Cancer Biology Graduate Program, University of Minnesota, Minneapolis, MN 55455 2 Center for Immunology, University of Minnesota, Minneapolis, MN 55455 3 Department of Urology, University of Minnesota, Minneapolis, MN 55455 4 Masonic Cancer Center, University of Minnesota, Minneapolis, MN 55455 Polyclonal antibodies are defined as a collection of antibodies directed against...
FAQs
Interpretation depends on cell-cycle status: Ki-67 binding identifies cells in active phases rather than treating the entire immune-cell population as proliferative. This distinction lets investigators separate expanding lymphocyte populations from cells that are present but not actively cycling. In infection studies, that separation clarifies whether an observed immune-cell increase reflects proliferation.
The FITC label converts antibody binding into an optical signal. After excitation, FITC emits green fluorescence, allowing Ki-67-positive nuclei or cells to be detected with flow cytometry or fluorescence microscopy. The biological information comes from Ki-67 recognition, while FITC supplies the visible readout needed to locate or measure proliferating cells.
Flow cytometry and fluorescence microscopy answer related but different questions with this reagent. Flow cytometry supports analysis across immune-cell populations, whereas microscopy shows fluorescence in cellular or nuclear context. Choosing between them depends on whether the study emphasizes population-level proliferation measurements or visual characterization of labeled cells within samples.
An analysis begins by applying the labeled antibody to an immune-cell sample, then detecting the resulting fluorescence with flow cytometry or microscopy. The readout is interpreted as evidence of Ki-67 expression and active cellular proliferation. In practice, the selected detection platform determines whether investigators emphasize population analysis or fluorescence localization.
During infection research, Ki67-FITC can be used to examine how lymphocyte proliferation changes after exposure to infectious stimuli. Comparing the detected proliferating-cell signal across infection-related conditions helps connect immune-cell expansion with infection progression. This provides a cellular measure of activation dynamics rather than relying only on broad disease observations.
Measurements obtained with this antibody can also track disease-associated changes and responses to treatment. A shift in the detected Ki-67-positive immune-cell population may indicate altered cellular activation or expansion, helping researchers relate therapeutic effects to immune dynamics. The same logic supports evaluation of vaccine-induced responses, where proliferation provides a readout of cellular engagement.