Large-scale Screening
Large-scale Screening - Related Videos
16:05
Large-Scale Screens of Metagenomic Libraries
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Cited by 9 •
2007
Metagenomic libraries archive large fragments of contiguous genomic sequences from microorganisms without requiring prior cultivation. Generating a ...
06:40
A Robust Method for the Large-Scale Production of Spheroids for High-Content Screening and Analysis Applications
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Cited by 4 •
2021
This protocol details a method for the production of three different types of spheroids in a manner that makes them suitable for large-scale high-content screening and analysis. In addition, examples are presented showing how they can be analyzed at spheroid and individual cell levels.
07:03
Large Scale Zebrafish-Based In vivo Small Molecule Screen
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Cited by 17 •
2010
Zebrafish has emerged as a powerful in vivo platform for phenotype-based drug screens and chemical genetic analysis. Here, we demonstrate a simple, practical method for large-scale screening of small molecules using zebrafish embryos.
12:47
A Technique to Screen American Beech for Resistance to the Beech Scale Insect (Cryptococcus fagisuga Lind.)
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Cited by 8 •
2014
Beech bark disease is initiated by feeding activities of the beech scale insect that create fungal entry points in the bark. Trees that are resistant to the scale insect are also disease resistant. Here we present the protocol we have developed to screen individual beech trees for beech scale resistance.
02:41
pH Scale
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2020
Hydronium and hydroxide ions are present both in pure water and in all aqueous solutions, and their concentrations are inversely proportional as determined by the ion product of water (Kw). The concentrations of these ions in a solution are often critical determinants of the solution’s properties and the chemical behaviors of its other solutes. Two different solutions can differ in their hydronium or hydroxide ion concentrations by a million, billion, or even trillion times. A common means of...
FAQs
Standardized assays apply the same testing conditions across many samples, making results more comparable within a study. This consistency helps researchers identify population-level patterns rather than confusing procedural differences with biological variation. In immunology and infection research, standardized measurements support comparisons of antibody, antigen, nucleic-acid, or immune-cell findings across individuals and sample groups.
The selected readout determines which aspect of infection or immunity becomes visible. Antibody measurements can indicate immune recognition, while antigen or nucleic-acid assays can support detection of infection-related material. Immune-cell response measurements provide another perspective on host activity. Using these distinct signals allows studies to compare exposure, infection, and immune responses more comprehensively.
Automation helps apply a consistent workflow across large sample sets and supports high-throughput analysis. Its value is not simply speed: standardized handling makes broad datasets easier to compare and analyze under controlled conditions. This enables researchers to examine patterns across populations and evaluate many candidate diagnostic or therapeutic measurements within the same general study framework.
A typical workflow begins by assembling a large set of samples, individuals, or biological measurements, followed by standardized assay testing. Automated processing may then support high-throughput analysis of the resulting signals. Researchers interpret the dataset to identify markers, conditions, exposure patterns, infected or immune individuals, or differences in host responses, depending on the study objective.
It is particularly useful when researchers need broad information across a population rather than observations from a small number of cases. Screening can help measure pathogen exposure, identify infected or immune individuals, and reveal population-level patterns. These findings support outbreak surveillance and epidemiological studies by showing how infection-related or immune signals are distributed across sampled groups.
Large screening datasets allow researchers to compare host responses and identify markers associated with infection or immunity. They can also support evaluation of diagnostic candidates and therapeutic candidates under controlled, standardized conditions. In vaccine research, broad measurements of immune responses can contribute evidence for comparing responses across individuals, while combined findings can inform infection-control strategies.