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Longitudinal Two-Photon Imaging of Dorsal Hippocampal CA1 in Live Mice
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Behavior
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JoVE Journal Behavior
Longitudinal Two-Photon Imaging of Dorsal Hippocampal CA1 in Live Mice

Longitudinal Two-Photon Imaging of Dorsal Hippocampal CA1 in Live Mice

DOI:
10.3791/59598-v

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09:34 min

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June 19, 2019

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, , , , , ,
1Dept. of Stress Neurobiology and Neurogenetics,Max Planck Institute of Psychiatry, 2Graduate School of Systemic Neurosciences,Ludwig Maximilians University, 3Max Planck Florida Institute for Neuroscience, 4Dept. of Neurobiology,Weizmann Institute of Science

Chapters

  • 00:04Title
  • 01:04Preparation of the Imaging Cannula
  • 02:11Implantation of the Imaging Cannula Over the Dorsal Hippocampus
  • 05:32Imaging Preparation
  • 06:16Cannula Imaging
  • 07:10Results: Longitudinal Imaging of Dendritic Structure and Dendritic Spines Dynamics
  • 08:50Conclusion

Summary

Automatic Translation

Automatic Translation

This method describes a chronic preparation that allows optical access to the hippocampus of living mice. This preparation can be used to perform longitudinal optical imaging of neuronal structural plasticity and activity-evoked cellular plasticity over a period of several weeks.

Tags

Keywords: Two-photon ImagingDorsal HippocampusCA1Live MiceLongitudinalMemory FormationMemory RecallAlzheimer’s DiseaseSurvival SurgeryImaging CannulaUV CuringDental DrillLambdaBregmaLidocainePeriosteum

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