Intravital Microscopy for Imaging Subcellular Structures in Live Mice Expressing Fluorescent Proteins

Andrius Masedunskas; Natalie Porat-Shliom; Muhibullah Tora; Oleg Milberg; Roberto Weigert

doi:10.3791/50558

Journal

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Biology

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Intravital Microscopy for Imaging Subcellular Structures in Live Mice Expressing Fluorescent Proteins

JoVE Journal
Biology

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JoVE Journal Biology

Intravital Microscopy for Imaging Subcellular Structures in Live Mice Expressing Fluorescent Proteins

23,537 Views

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08:23 min

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September 01, 2013

DOI:

10.3791/50558-v

DOI:

10.3791/50558-v

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08:23 min

September 01, 2013

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3 Views

Andrius Masedunskas^1,2, Natalie Porat-Shliom¹, Muhibullah Tora¹, Oleg Milberg^1,3, Roberto Weigert¹

¹Intracellular Membrane Trafficking Unit, Oral and Pharyngeal Cancer Branch National Institute of Dental and Craniofacial Research,National Institutes of Health, ²Department of Biology,University of North Carolina at Chapel Hill , ³Department of Chemical & Biochemical Engineering and Department of Biomedical Engineering,Rutgers University

Summary

Intravital microscopy is a powerful tool that enables imaging various biological processes in live animals. In this article, we present a detailed method for imaging the dynamics of subcellular structures, such as the secretory granules, in the salivary glands of live mice.