Membrane Oxygenator
Membrane Oxygenator - Related Videos
06:41
Veno-Venous Extracorporeal Membrane Oxygenation in a Mouse
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Cited by 10 •
2018
Here we present a protocol describing the technique of veno-venous extracorporeal membrane oxygenation (ECMO) in a non-intubated, spontaneously breathing mouse. This murine model of ECMO can be effectively implemented in experimental studies of acute and end-stage lung diseases.
09:56
Determination of Mitochondrial Membrane Potential and Reactive Oxygen Species in Live Rat Cortical Neurons
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Cited by 230 •
2011
We demonstrate application of the fluorescence indicator, TMRM, in cortical neurons to determine the relative changes in TMRM fluorescence intensity before and after application of a specific stimulus. We also show application of the fluorescence probe H2DCF-DA to assess the relative level of reactive oxygen species in cortical neurons.
09:08
Detecting Reactive Oxygen Species
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2023
Reactive oxygen species are chemically active, oxygen-derived molecules capable of oxidizing other molecules. Because of their reactive nature, there are many deleterious effects associated with unchecked ROS production, including structural damage to DNA and other biological molecules. However, ROS can also be mediators of physiological signaling. There is accumulating evidence that ROS play significant roles in everything from activation of transcription factors to the mediation of...
08:58
Cerebral Blood Oxygenation Measurement Based on Oxygen-dependent Quenching of Phosphorescence
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Cited by 28 •
2011
We present an experimental procedure for measuring the partial pressure of oxygen (pO2) in cerebral vasculature based on oxygen-dependent quenching of phosphorescence. Animal preparation and imaging procedures were outlined for both large field of view CCD-based imaging of pO2 in rats and 2-photon excitation based imaging of pO2 in mice.
03:40
Point-of-Care Ultrasound for Peripheral Veno-Arterial Extracorporeal Membrane Oxygenation Without Left Ventricular Venting
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Cited by 1 •
2025
This protocol outlines the use of point-of-care ultrasound (POCUS) to monitor patients on peripheral veno-arterial extracorporeal membrane oxygenation (V-A ECMO) without left ventricular venting. It evaluates left ventricular distension, intracardiac or aortic root thrombus, and identifies useful cardiac POCUS parameters for weaning from V-A ECMO.
FAQs
Gas exchange depends on differences in partial pressure across the semipermeable membrane. An oxygen-rich gas mixture creates the gradient that drives oxygen toward the blood, while carbon dioxide moves from blood toward the gas side. Because the two streams remain separated, the device transfers gases without direct blood-gas mixing, supporting oxygen delivery and carbon dioxide removal.
The membrane provides the physical interface between circulating blood and the oxygen-rich gas mixture while keeping them apart. This separation enables diffusion-based gas transfer, but blood still contacts an artificial material. That contact is important in immunology and infection research because it can be examined alongside inflammatory and coagulation responses during extracorporeal support.
Blood-material interactions, inflammation, and coagulation are key processes that may influence extracorporeal support. Infection-related lung injury adds another clinically relevant factor because it can affect the need for support and the patient's response. Studying these processes together helps connect device operation with tissue oxygenation and broader patient outcomes.
Its value in this field comes from providing a clinically relevant setting for examining how blood interacts with an artificial surface during support. Researchers can use that context to investigate inflammatory and coagulation responses, as well as the effects of infection-related lung injury. These links help interpret why extracorporeal support may produce different outcomes across clinical situations.
In extracorporeal membrane oxygenation, the device helps maintain gas exchange when severe respiratory failure compromises normal lung function. During cardiopulmonary bypass, it supports gas exchange during surgery. In both settings, blood passes along one side of the membrane while the gas mixture passes on the other, allowing oxygenation and carbon dioxide removal outside the body.
Studies can assess how extracorporeal support maintains tissue oxygenation while removing carbon dioxide, then relate those functions to biological responses. The system also allows investigation of blood-material interactions, inflammation, coagulation, and infection-related lung injury. Together, these observations provide context for understanding device performance and factors associated with patient outcomes.