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JoVE Journal
Biology

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DNA Methylation
 

DNA Methylation: Bisulphite Modification and Analysis

Article doi: 10.3791/3170
October 21st, 2011

Summary October 21st, 2011

The gold standard for DNA methylation analysis is genomic sequencing of bisulphite converted DNA. This method takes advantage of the increased sensitivity of cytosine compared with 5-methylcytosine (5-MeC) to bisulphite deamination under acidic conditions. Unmethylated cytosines can be distinguished from methylated cytosines after PCR amplification of the target genomic DNA.

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