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JoVE Journal

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DNA Methylation

DNA Methylation: Bisulphite Modification and Analysis

Article DOI: 10.3791/3170-v 12:34 min October 21st, 2011
October 21st, 2011



The gold standard for DNA methylation analysis is genomic sequencing of bisulphite converted DNA. This method takes advantage of the increased sensitivity of cytosine compared with 5-methylcytosine (5-MeC) to bisulphite deamination under acidic conditions. Unmethylated cytosines can be distinguished from methylated cytosines after PCR amplification of the target genomic DNA.


DNA Methylation Bisulphite Modification Analysis Epigenetics Gene Function DNA Sequence DNA Methylation Patterns 5-MeC Gene Expression Machinery CpG Dinucleotides Genomic Sequences Gene Promoters DNA Methylation Analysis Bisulphite Conversion Cytosine Deamination Sodium Bisulphite
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