Murine Lung Isolation
Murine Lung Isolation - Related Videos
09:04
Isolation of Mouse Lung Dendritic Cells
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Cited by 17 •
2011
A highly purified preparation of mouse lung dendritic cells is described. Specific emphasis is given to the isolation of conventional dendritic cell subset.
06:14
Isolation of Alveolar Type II Epithelial Cells from Neonatal, Juvenile, and Adult Murine Lungs Adaptable to Infectious Experimental Settings
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2025
This protocol presents a flow cytometry-based method to isolate viable alveolar type II epithelial cells (AECII) from neonatal, juvenile, or adult murine lungs (infected or non-infected). This approach enables downstream molecular and/or functional studies of AECII to identify their roles during lung inflammation and infection.
09:47
Isolation of CD146+ Resident Lung Mesenchymal Stromal Cells from Rat Lungs
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Cited by 3 •
2016
This protocol describes an isolation technique for obtaining primary lung resident mesenchymal stromal cells from rats, through the use of enzymatic digestion, density gradient separation, plastic adherence and CD146+ magnetic bead selection.
05:54
An In Vitro Method to Identify Innate and Adaptive Immune Cells Residing in Murine Lungs
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2025
This video demonstrates an in vitro technique to identify immune cells residing in murine lungs. After isolating target immune cells from murine lung tissue, they are surface-labeled using a cocktail of fluorophore-tagged antibodies against specific markers on innate and adaptive immune cells. Additionally, monocytes, macrophages, and dendritic cells are labeled using an intracellular marker. The various immune cell types are then identified using flow cytometry.
08:56
Isolation of Murine Embryonic Hemogenic Endothelial Cells
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Cited by 11 •
2016
Hematopoietic stem and progenitor cells (HSPC) derive from specialized (hemogenic) endothelial cells during development, yet little is known about the process by which some endothelial cells specify to become blood forming. We demonstrate a flow-cytometry based method allowing simultaneous isolation of hemogenic endothelial cells and HSPC from murine embryonic tissues.
FAQs
The preparation makes it possible to examine respiratory structures alongside resident cells and immune components rather than treating the lung only as a bulk sample. This distinction supports questions about where host responses occur and which cellular populations are present. In infection studies, it helps connect pulmonary tissue changes with local immunological findings.
Mechanical and enzymatic dissociation converts prepared lung tissue into a viable single-cell suspension when the experiment requires cell-level analysis. That format enables researchers to characterize cellular populations, including immune components, with assays such as flow cytometry. Choosing dissociation shifts the analysis from tissue organization toward cell composition and immune-cell recruitment.
Because the preparation exposes pulmonary tissue and its associated cellular components, investigators can examine resident immune populations while also assessing immune-cell recruitment during disease. This is especially relevant when infection or inflammation changes the cellular makeup of the lung. The result is a tissue-centered view of host response rather than an analysis detached from pulmonary structures.
The workflow begins by exposing the thoracic cavity and separating the lungs from surrounding tissues. The resulting preparation can then be retained for tissue-based examination or mechanically and enzymatically dissociated when a viable single-cell suspension is needed. This branching workflow allows the same general preparation to support microscopy, molecular assays, or cell-based measurements.
Researchers apply this preparation when they need to study host responses to respiratory pathogens directly within pulmonary tissue. It supports investigation of immune-cell recruitment, inflammation, tissue injury, and repair. These applications connect immunological mechanisms with changes in the infected or inflamed lung, making the preparation relevant to models of pulmonary disease.
The appropriate assay depends on whether the study emphasizes tissue organization, cellular composition, or molecular changes. Intact preparations can support microscopy, while dissociated tissue provides a viable suspension for flow cytometry. Molecular assays add another layer of analysis, allowing researchers to relate structural and cellular observations to pulmonary immune responses.
Isolated lungs can provide evidence about the presence of resident cells, the recruitment of immune cells, and the extent of inflammation after respiratory challenge. They also support evaluation of tissue injury and repair. Examining these outcomes together helps researchers relate cellular mechanisms to broader pulmonary disease and immunological consequences.