Non Invasive Detection
Non Invasive Detection - Related Videos
08:01
Detection of Invasive Pulmonary Aspergillosis in Haematological Malignancy Patients by using Lateral-flow Technology
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Cited by 50 •
2012
A rapid and accurate point-of-care test for invasive pulmonary aspergillosis is presented. It takes advantage of lateral-flow technology using a specific monoclonal antibody that binds to an Aspergillus antigen secreted during pulmonary infections. The assay is compatible with serum and brochoalveolar lavage and represents a novel adjunct test for disease diagnosis.
10:21
In Vivo Imaging Systems (IVIS) Detection of a Neuro-Invasive Encephalitic Virus
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Cited by 11 •
2012
Utilizing luciferase and in vivo imaging systems (IVIS) as a novel means to identify disease endpoints before clinical developments occur. IVIS has allowed us to visualize in real time the invasion of encephalitic viruses over multiple days, providing a more accurate disease model for future study. It has also allowed us to identify the potential protective features of antivirals and vaccines faster than currently utilized animal models. The capability to utilize individual animals over...
06:20
Detection of Enterohemorrhagic Escherichia Coli Colonization in Murine Host by Non-invasive In Vivo Bioluminescence System
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Cited by 1 •
2018
A detailed protocol of a mouse model for enterohemorrhagic E. coli (EHEC) colonization by using bioluminescence-labeled bacteria is presented. The detection of these bioluminescent bacteria by a non-invasive in vivo imaging system in live animals can advance our current understanding of EHEC colonization.
07:42
Labeling Stem Cells with Fluorescent Dyes for non-invasive Detection with Optical Imaging
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Cited by 33 •
2008
This video shows techniques for labeling of human embryonic stem cells and mesenchymal stem cells with fluorescent dyes. This technique can be used for an in vivo tracking of transplanted stem cells with optical imaging and for histopathological correlations with fluorescence microscopy.
10:16
Chick Heart Invasion Assay for Testing the Invasiveness of Cancer Cells and the Activity of Potentially Anti-invasive Compounds
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Cited by 4 •
2015
Here, we present a protocol to study the invasion of tumor cells into living normal tissue fragments in three dimensions. This organ culture technique is mainly applied to test potentially anti-invasive drugs in vitro.
FAQs
Molecular recognition selects a pathogen-derived molecule or host immune biomarker, while nucleic-acid amplification increases the detectable signal when the target is genetic material. Together, these mechanisms convert a biological event into a measurable result without tissue removal. This combination can support earlier assessment of infection and make repeated measurements useful for following changes over time.
The approach can target three broad signal classes: pathogen-derived molecules, host immune biomarkers, and physiological signals. Pathogen signals provide information about infection-associated material, whereas immune biomarkers reflect the host response. Physiological signals add information from intact tissues or body processes. Selecting among these signals helps align the measurement with infection status, immune activity, or both.
Sensors and imaging systems provide ways to translate biological activity into an observable readout without surgical access. A sensor converts recognition of a relevant biological signal into a measurable output, while imaging can examine signals in intact tissues. These mechanisms broaden detection beyond removed samples and can support monitoring when the biological state changes over time.
Because the approach avoids surgical entry and tissue removal, measurements can be repeated with less discomfort and sampling burden. A series of observations can therefore track pathogen-associated signals, immune biomarkers, or physiological changes across time rather than relying on a single measurement. This is relevant for following infection assessment, immune responses, and changes associated with treatment or vaccination.
A practical workflow begins by selecting the biological question and the corresponding signal, such as a pathogen-derived molecule, host immune biomarker, or physiological change. Researchers then use an accessible specimen or intact tissue and choose molecular recognition, nucleic-acid amplification, imaging, or sensor-based conversion. The resulting readout is interpreted in relation to infection status or immune activity.
It is particularly useful when investigators need repeated observations during treatment or after vaccination. Measurements can follow pathogen-associated signals, host immune biomarkers, or physiological responses while reducing the burden associated with sampling. This longitudinal perspective helps researchers assess how biological states change over time and supports evaluation of treatment effects or vaccine-related immune responses.