Rodent Infection Models
Rodent Infection Models - Related Videos
10:29
Principles of Rodent Surgery for the New Surgeon
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Cited by 56 •
2011
Before attempting surgery, a new surgeon should have training in basic surgical techniques and concepts. This article will present basic surgical considerations with an emphasis on rodents.
05:58
Retinal Detachment Model in Rodents by Subretinal Injection of Sodium Hyaluronate
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Cited by 44 •
2013
Creating experimental retinal detachments with a reproducible and sustained height of detachment, and without subretinal hemorrhage, is important for studying the pathophysiology of photoreceptor cell loss in retinal disease and evaluating potential therapeutic interventions. Here, we report such a method in detail.
12:30
Use of Galleria mellonella as a Model Organism to Study Legionella pneumophila Infection
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Cited by 108 •
2013
The larva of the wax moth Galleria mellonella was recently established as an in vivo model to study Legionella pneumophila infection. Here, we demonstrate fundamental techniques to characterize the pathogenesis of Legionella in the larvae, including inoculation, measurement of bacterial virulence and replication as well as extraction and analysis of infected hemocytes.
09:40
Hemodynamic Characterization of Rodent Models of Pulmonary Arterial Hypertension
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Cited by 35 •
2016
Pulmonary arterial hypertension (PAH) is a disease of pulmonary arterioles that leads to their obliteration and the development of right ventricular failure. Rodent models of PAH are critical in understanding the pathophysiology of PAH. Here we demonstrate hemodynamic characterization, with right heart catheterization and echocardiography, in the mouse and rat.
12:13
Considerations for Rodent Surgery
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2016
Source: Kay Stewart, RVT, RLATG, CMAR; Valerie A. Schroeder, RVT, RLATG. University of Notre Dame, IN The Guide for the Care and Use of Laboratory Animals1 dictates that rodent survival surgery be performed aseptically. Aseptic technique utilizes specific practices that minimize the contamination of the surgical site, including patient preparation, surgeon preparation, sterilization of instruments and other supplies, and the use of a clean and controlled environment. Presurgical planning,...
FAQs
Outcomes depend strongly on the inoculated pathogen, delivery route, and dose, because these variables shape how exposure reaches host tissues and how much infectious challenge the animal experiences. Keeping them defined allows investigators to compare pathogen burden, cytokine production, immune-cell activity, and tissue pathology across experimental conditions, rather than attributing differences to uncontrolled exposure.
These models can separate early innate responses from later adaptive immunity by examining cytokine production and immune-cell activity alongside pathogen burden and tissue pathology. That comparison helps connect a measurable defense response with its effect on infection and tissue damage. It is especially useful when researchers want to determine which arm of immunity contributes to disease control or severity.
Rodent findings require cautious interpretation because rodent and human biology are not identical. A response that explains pathogen control, cytokine production, or pathology in an animal may not transfer directly to people. For that reason, infection studies are most informative when their conclusions are considered alongside complementary experimental approaches rather than treated as complete predictions of human disease.
A typical study begins by selecting a pathogen and establishing the intended inoculation route and dose. After exposure, investigators assess pathogen burden, cytokine production, immune-cell activity, and tissue pathology. Linking the initial challenge to these readouts creates a structured way to follow infection and host response under controlled experimental conditions.
Rodent infection models support vaccine and antimicrobial evaluation by showing whether an intervention changes infection-related measurements. Investigators can compare pathogen burden, immune activity, cytokine production, or tissue pathology between experimental conditions. These outcomes indicate whether a treatment is associated with improved pathogen control or reduced disease-related damage, while also revealing immune mechanisms that may explain its effects.
Researchers can vary defined experimental conditions and then relate those conditions to pathogen burden, cytokine production, immune-cell activity, and tissue pathology. This approach helps identify links between the infectious challenge, host defense, and tissue damage. In immunology and infection research, such comparisons clarify mechanisms that may increase or limit disease severity.