Tissue Slice Culture
Tissue Slice Culture - Related Videos
04:13
Generating and Maintaining Slice Cultures from Human Glioblastoma Tissue
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2025
The video demonstrates the generation and maintenance of organotypic slice cultures from glioblastoma tissue. The isolated tissue is embedded in an agarose matrix, and thin sections are obtained using a vibratome. These sections are then placed on permeable membrane inserts within a microplate and cultured in a slice culture maintenance media for long-term maintenance.
09:17
Ex Utero Electroporation and Organotypic Slice Culture of Mouse Hippocampal Tissue
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Cited by 4 •
2015
Here we present a protocol providing a tool to examine regulatory mechanisms of specific genes during hippocampal development. Employing ex utero electroporation and organotypic slice culture allows the up- and down-regulation of the expression of genes of interest in single cells and follow their fate during development.
08:20
Organotypic Hippocampal Slice Cultures
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Cited by 52 •
2011
We describe a method to prepare organotypic hippocampal slices that can be easily adapted to other brain regions. Brain slices are laid on porous membranes and culture media is allowed to form an interface. This method preserves the gross architecture of the hippocampus for up to 2 weeks in culture.
05:33
Lung-tumor Tissue Explant Culture: A Procedure to Generate and Culture Precision-cut Lung Tumor Slices
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2023
Organotypic primary tissue explant cultures, which include precision-cut slices, represent the three-dimensional (3-D) tissue architecture as well as the multicellular interactions of native tissue. This video describes the culturing of murine lung tumor-derived tissue slices using a rotating incubation unit, a system that enables intermittent exposure of tissues to oxygen and nutrients. The protocol describes the methodology to generate and culture precision-cut lung tumor slices for drug...
05:02
Cerebellum Dissection and Slice Preparation: A Method to Generate Tissue Slices from Mouse Cerebellum
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2025
This video describes a method to generate tissue slices from the mouse cerebellum. The cerebellar slices can be used for organotypic culturing that provides insights into neurodevelopmental mechanisms, including myelination dynamics.
FAQs
Viability depends on diffusion through the slice, while culture media supply nutrients and gas exchange supports oxygen availability. Because the tissue section is thin, these conditions can sustain resident immune, stromal, and parenchymal cells without removing them from their local arrangement. Maintaining this balance is essential for interpreting responses to infection or inflammation ex vivo.
Native architecture preserves local contact among immune, stromal, and parenchymal cells, allowing investigators to examine responses within their tissue context. This organization can provide information that isolated cell cultures cannot, particularly when studying leukocyte behavior, cytokine production, or tissue injury. The resulting observations connect cellular activity with changes occurring across the tissue section.
Media composition, nutrient delivery, gas exchange, and diffusion determine whether cells remain viable and responsive. Slice thickness also matters because nutrients and oxygen must reach cells through the tissue. If these conditions are not controlled, reduced viability could complicate interpretation of cytokine production, leukocyte behavior, pathogen growth, or tissue injury after experimental stimulation.
This approach occupies an intermediate position between reductionist cell cultures and whole-animal experiments. It retains tissue architecture and local cell interactions while allowing controlled exposure to pathogens, antigens, or inflammatory stimuli. Consequently, researchers can examine tissue-level responses with more experimental control than an animal study and more structural context than an isolated-cell system.
Researchers prepare thin sections of an organ or other tissue, maintain them in controlled culture conditions, and expose them to a selected pathogen, antigen, or inflammatory stimulus. They then assess outcomes such as leukocyte behavior, cytokine production, pathogen growth, or tissue injury. This workflow enables direct comparison of tissue responses under defined experimental conditions.
Tissue slices support host-pathogen analysis by showing how pathogens grow and how resident cells respond within tissue. They also allow evaluation of inflammatory effects and potential therapeutic responses, while human tissue applications can provide context relevant to human disease. Measurements of cytokines, leukocyte behavior, and tissue injury help link immune activity with tissue consequences.