Acute Organ Injury
Acute Organ Injury - Related Videos
09:09
Ischemia-reperfusion Model of Acute Kidney Injury and Post Injury Fibrosis in Mice
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Cited by 141 •
2013
We describe models of moderate and severe ischemia-reperfusion-induced kidney injury in which the mice undergo unilateral renal pedicle clamping followed by simultaneous or delayed contralateral nephrectomy, respectively. These models consistently give rise to renal dysfunction and post-injury fibrosis, but injury severity and survival are dependent on mouse background, age and surgical equipment.
07:55
Pressure Controlled Ventilation to Induce Acute Lung Injury in Mice
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Cited by 7 •
2011
A murine model for ventilator induced lung injury is an important tool to study an acute lung injury in vivo. Here, we report an easy applicable in situ model for acute lung injury using high-pressure mechanical ventilation to induce acute failure of the lung.
10:45
Histological Analyses of Acute Alcoholic Liver Injury in Zebrafish
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Cited by 27 •
2017
This protocol describes histological analyses of the livers from zebrafish larvae that have been treated with 2% ethanol for 24 h. Such acute ethanol treatment results in hepatic steatosis and swelling of the hepatic vasculature.
07:58
Nephrotoxin Microinjection in Zebrafish to Model Acute Kidney Injury
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Cited by 8 •
2016
Renal injuries incurred from nephrotoxins, which include drugs ranging from antibiotics to chemotherapeutics, can result in complex disorders whose pathogenesis remains incompletely understood. This protocol demonstrates how zebrafish can be used for disease modeling of these conditions, which can be applied to the identification of renoprotective measures.
04:10
Non-Invasive Endotracheal Administration of Lipopolysaccharide to Induce Acute Lung Injury in Rodents
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2025
In this study, a non-invasive endotracheal instillation technique is demonstrated to develop ALI in rats using a basic laryngoscope to ensure precise LPS delivery to the lungs. The LPS is instilled via an endotracheal tube using a laryngoscope. The magnitude of lung injury is evaluated at multiple time points.
FAQs
These insults disrupt normal cellular and tissue conditions through different immediate pathways, but they can converge on impaired energy production and altered microcirculation. As cells lose functional stability, structural damage and cell death may follow. Identifying the initiating insult helps clinicians target its removal and anticipate secondary damage that could worsen organ dysfunction.
Reduced cellular energy production limits the ability of affected cells to maintain normal function, while altered microcirculation can restrict effective tissue support. Together, these changes may intensify local injury and promote progression toward organ failure. Their importance explains why early recognition and supportive care focus on preserving remaining function and limiting additional tissue damage.
An excessive inflammatory response can extend damage beyond the site or mechanism that first affected the organ. Inflammation may contribute to further cellular dysfunction, cell death, and systemic complications, increasing the risk that injury will progress. This mechanism makes prompt treatment of the underlying cause and careful supportive management important for limiting secondary effects.
Clinicians combine clinical assessment with laboratory biomarkers and imaging to recognize patterns associated with the affected organ. Each source contributes different information about altered function or structure, while their combined interpretation can also suggest the underlying cause. This evaluation supports timely intervention, helps estimate severity, and provides a basis for monitoring progression or recovery.
Management begins by recognizing the injury pattern, identifying the underlying insult, and removing or addressing that insult when possible. Supportive care then helps preserve organ function while clinicians monitor for progression and systemic complications. Early action is important because limiting secondary damage can influence prognosis and improve the likelihood of recovery.
Clinical observations and outcome data help researchers examine how ischemia, infection, toxins, trauma, inflammation, energy disruption, and microcirculatory changes affect tissue. Biomarkers and imaging provide ways to characterize injury and follow its course. This information informs investigations into strategies that protect damaged tissue, limit progression, and support repair after the initial insult is controlled.