Anterior Chamber Reinflation

Anterior chamber reinflation is an ophthalmic procedure that restores the normal depth and pressure of the eye’s anterior chamber after it becomes shallow or collapses, helping protect vision and ocular structures. Clinicians typically introduce sterile balanced salt solution or an ophthalmic viscoelastic through a small corneal entry, re-forming the space between the cornea and iris and repositioning displaced tissues. The technique is used after eye surgery, trauma, or wound leakage to support healing, protect the corneal endothelium, evaluate wound integrity, and reduce complications associated with a persistently flat anterior chamber.

Anterior Chamber Reinflation - Related Videos

Research

JoVE Journal - Biology
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In Vivo Imaging of Liver Spheroids Engrafted in the Anterior Chamber of the Mouse Eye

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2024

Here, we describe a platform that allows noninvasive in vivo imaging of liver spheroids engrafted in the anterior chamber of the mouse eye. The workflow spans from generating spheroids from primary liver cells to transplantation into the mouse eye and in vivo imaging at cellular resolution by confocal microscopy.

Research

JoVE EoE - Pancreatic Cancer

Grafting Pancreatic Islets: A Method to Implant Pancreatic Islets into the Anterior Chamber of the Mouse Eye for In Vivo Imaging

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2023

This video describes the protocol for grafting human pancreatic islets into the anterior chamber of the mouse’s eye to monitor revascularization of human islet grafts in vivo. It also helps to understand the contribution of recipient versus donor cells in promoting the encapsulation and vascularization of the graft.

Real Time In Vivo Tracking of Thymocytes in the Anterior Chamber of the Eye by Laser Scanning Microscopy

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Cited by 2 •

2018

The goal of the protocol is to show longitudinal intravital real-time tracking of thymocytes by laser scanning microscopy in thymic implants in the anterior chamber of the mouse eye. The transparency of the cornea and vascularization of the graft allows for continuously recording progenitor cell recruitment and mature T-cell egress.

Transplantation into the Anterior Chamber of the Eye for Longitudinal, Non-invasive In vivo Imaging with Single-cell Resolution in Real-time

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Cited by 31 •

2013

A new approach combining intraocular transplantation and confocal microscopy enables longitudinal, non-invasive real-time imaging with single-cell resolution within grafted tissues in vivo. We demonstrate how to transplant pancreatic islets into the anterior chamber of the mouse eye.

Upright Imaging of Drosophila Egg Chambers

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Cited by 1 •

2015

The upright imaging method described in this protocol allows for the detailed visualization of the poles of a developing Drosophila melanogaster egg. This end-on view provides a new perspective into the arrangements and morphologies of multiple cell types in the follicular epithelium.

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