Cell Sheet Transplantation
Cell Sheet Transplantation - Related Videos
05:57
ADSC-sheet Transplantation to Prevent Stricture after Extended Esophageal Endoscopic Submucosal Dissection
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Cited by 14 •
2017
This study reports a successful method of endoscopical adipose tissue-derived stromal cell (ADSC)-sheet transplantation for esophageal stricture prevention after an extended endoscopic submucosal dissection (ESD) in a swine model.
09:24
Tissue Engineering: Construction of a Multicellular 3D Scaffold for the Delivery of Layered Cell Sheets
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Cited by 2 •
2014
For creation of highly organized structures of complex tissue, one must assemble multiple material and cell types into an integrated composite. This combinatorial design incorporates organ-specific layered cell sheets with two distinct biologically-derived materials containing a strong fibrous matrix base, and endothelial cells for enhancing new vessels formation.
09:24
Molecular Imaging to Target Transplanted Muscle Progenitor Cells
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Cited by 3 •
2013
A non-invasive means to evaluate the success of myoblast transplantation is described. The method takes advantage of a unified fusion reporter gene composed of genes whose expression can be imaged with different imaging modalities. Here, we make use of a fluc reporter gene sequence to target cells via bioluminescence imaging.
08:06
Creation and Transplantation of an Adipose-derived Stem Cell (ASC) Sheet in a Diabetic Wound-healing Model
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Cited by 32 •
2017
Adipose-derived stem cells (ASCs) are easily isolated and harvested from the fat of normal rats. ASC sheets can be created using cell-sheet engineering and can be transplanted into Zucker diabetic fatty rats exhibiting full-thickness skin defects with exposed bone and then covered with a bilayer of artificial skin.
08:15
Cervical Heterotopic Mouse Heart Transplantation using End-to-side Anastomosis Technique to Study Transplant Immunology
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2026
This protocol presents a simplified, end-to-side suture anastomosis technique for murine cervical heart transplantation, providing a robust and reproducible model for studying transplant immunology.
FAQs
The temperature-responsive surface allows cultured cells to be released by lowering temperature rather than applying enzymes. This preserves cell-cell connections and extracellular matrix within the harvested layer, retaining biological properties that may be lost when cell associations are disrupted. The result is a cohesive, biologically intact graft suitable for transfer to damaged tissue.
The preserved extracellular matrix travels with viable cells instead of being removed before transplantation. Cell-cell connections also remain within the cohesive layer. Consequently, the transplanted material delivers both living cells and matrix components to the target site, supporting the regenerative strategy without reducing the graft to a suspension of individually separated cells.
Cell sheets may be applied directly to a target site or layered to create a thicker cellular construct. This flexibility provides an alternative to relying primarily on synthetic scaffolds in cell-based therapies. The approach therefore combines living cells with their retained matrix while allowing the graft arrangement to be adapted to the repair setting.
Cells are first cultured on a temperature-responsive surface until they form a cohesive layer. Lowering the culture temperature then releases the sheet without enzyme treatment. The intact layer can subsequently be transferred directly to damaged tissue or combined with other sheets by layering, depending on the intended regenerative application.
The technique has potential across several regenerative medicine settings, including injuries and diseases affecting epithelial, cardiac, and periodontal tissues. It may also be relevant to other damaged tissues where delivery of viable cells together with extracellular matrix is desirable. These applications reflect the method's broad tissue-repair focus rather than a single disease indication.
A transplanted sheet delivers viable cultured cells together with preserved extracellular matrix and cell-cell connections. These components are transferred as a cohesive layer to the target site, where they can contribute to the intended tissue-repair strategy. The method's potential value lies in providing cellular and matrix material while reducing reliance on synthetic scaffolds.