Cfse Proliferation
Cfse Proliferation - Related Videos
04:10
The Use of Carboxyfluorescein Diacetate Succinimidyl Ester (CFSE) to Monitor Lymphocyte Proliferation
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Cited by 214 •
2010
CFSE covalently labels long-lived intracellular molecules with the fluorescent dye, carboxyfluorescein. As such, when a CFSE-labeled cell divides, its progeny have half the amount of fluorescence, which can thereby be used to assess cell division. This article describes the procedures typically used for labeling mouse lymphocytes with CFSE.
10:57
Cell Cycle Analysis: Assessing CD4 and CD8 T Cell Proliferation After Stimulation Using CFSE Staining and Flow Cytometry
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2023
Source: Perchet Thibaut1,2,3, Meunier Sylvain1,2,3, Sophie Novault4, Rachel Golub1,2,3 1 Unit for Lymphpoiesis, Department of Immunology, Pasteur Institute, Paris, France 2 INSERM U1223, Paris, France 3 Université Paris Diderot, Sorbonne Paris Cité, Cellule Pasteur, Paris, France 4 Flow Cytometry Platfrom, Cytometry and Biomarkers UtechS, Center for Translational Science, Pasteur Institute, Paris, France The cell cycle is a universal process of life. During the cell cycle, a cell undergoes...
09:49
Antigen Specific In Vivo Killing Assay using CFSE Labeled Target Cells
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Cited by 39 •
2010
Many infections elicit a strong CTL response, but occasionally, the quantity of responding cells does not correlate to control of the pathogen1. One measure of CTL quality is their ability to kill specifically2. CFSE labeling of target cells can be used to investigate this CTL response quality in vivo3,4.
02:46
An In Vitro Assay for Evaluating the Immunomodulatory Impact of Monocytes on Leukocyte Proliferation
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2025
This video demonstrates an in vitro assay to explore the immunomodulatory influence of mesenchymal stem cells (MSCs) on effector T cell proliferation. The assay includes co-culturing MSCs with PBMCs to induce the monocyte population to acquire an immunomodulatory phenotype, leading to the secretion of interleukin-10 (IL-10). Following incubation with activated CD4+ T cells, the induced monocytes effectively suppress T cell proliferation.
11:54
Identification and Isolation of Slow-Dividing Cells in Human Glioblastoma Using Carboxy Fluorescein Succinimidyl Ester (CFSE)
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Cited by 18 •
2012
This video protocol demonstrates the application of the fluorescent dye carboxyfluorescein succinimidyl ester (CFSE) for the identification and separation of different sub-populations of cells in human glioblastoma based on frequency of cell division.
FAQs
As labeled cells divide, their intracellular fluorescent material is distributed between daughter cells, producing an approximately twofold reduction in fluorescence with each generation. Flow cytometry can therefore separate successive intensity levels and associate them with division history. This makes it possible to distinguish cells that have remained undivided from populations that have undergone multiple rounds of proliferation.
The dye forms stable fluorescent bonds with intracellular proteins after entering the cell. That retention preserves a measurable signal as cells progress through division, while the signal is apportioned between daughter cells. Without a persistent intracellular label, fluorescence would provide less reliable information about successive generations and the extent of proliferation in rapidly dividing populations.
CFSE analysis can indicate both the proportion of cells that respond and the number of divisions completed by those cells. These are different aspects of proliferation: one describes the size of the responding fraction, while the other describes its division history. Together, they provide a more detailed view of cellular responses than a simple activated-versus-inactive classification.
A typical workflow labels the cell population with CFSE, allows the cells to undergo the experimental condition being studied, and then measures fluorescence by flow cytometry. The resulting fluorescence distribution is interpreted as successive cellular generations. This workflow enables investigators to quantify responding cells and determine how many divisions occurred during the assay.
In antigen-specific studies, the assay can reveal whether a lymphocyte population responds to the antigen being examined and how extensively the responding cells divide. Measuring both response frequency and division number helps characterize the strength of the cellular immune response. This supports investigations of immune function and disease mechanisms in medicine.
The method allows investigators to compare lymphocyte proliferation under conditions involving immunosuppressive treatments or cellular therapies. Changes in the fraction of responding cells or in their division history provide quantitative evidence of altered cellular behavior. These measurements support therapeutic development by showing whether an intervention modifies immune activation or the performance of relevant cell populations.