Fluorescent Transgenic Animals
Fluorescent Transgenic Animals - Related Videos
05:51
Culturing Fluorescent Excitatory Neurons from a Transgenic Mouse Pup Brain
0 Views •
2025
The video illustrates a technique for culturing fluorescent GABAergic and glutamatergic neurons from the cortex and hippocampal tissues of transgenic mouse pups. The process includes obtaining purified single-cell suspensions of neurons, sorting GABAergic neurons based on their fluorescence protein expression, and seeding them onto poly-L-lysine-coated plates to facilitate attachment and cell proliferation.
02:48
Fluorescence Imaging of Calcium Dynamics at Neuromuscular Junctions in the Diaphragm of a Transgenic Mouse
0 Views •
2025
Source: Heredia, D. J., et. al. Ex Vivo Imaging of Cell-specific Calcium Signaling at the Tripartite Synapse of the Mouse Diaphragm. J. Vis. Exp. (2018) This video demonstrates fluorescence imaging of calcium dynamics at neuromuscular junctions (NMJs) in a transgenic mouse expressing genetically encoded calcium indicators. A NJM is identified by fluorescently tagged acetylcholine receptors in the diaphragm with the phrenic nerve. An image splitter is used to visualize multiple signals...
08:15
Tracking Dynamics of Muscle Engraftment in Small Animals by In Vivo Fluorescent Imaging
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Cited by 4 •
2009
We describe an in vivo fluorescence imaging protocol to monitor muscle regeneration by GFP-labeled myoblasts after transplantation into skeletal muscles of both healthy and dystrophic mice. This protocol can be adapted to study muscle regeneration by transplantation of other types of cells and in other muscular conditions as well.
12:09
Generation of Stable Transgenic C. elegans Using Microinjection
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Cited by 93 •
2008
This video demonstrates the technique of microinjection into the gonad of C. elegans to create transgenic animals.
03:39
An In Vivo Assay to Detect Mitophagy in Transgenic Nematodes
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2025
This video demonstrates an in vivo assay to study mitophagy in Caenorhabditis elegans. Upon exposing the nematodes to a mitophagy-inducing drug, the occurrence of mitophagy is assessed by co-localization between DCT-1, a mitophagy receptor on the damaged mitochondria, and the autophagosomal membrane protein LGG-1.
FAQs
Fluorescent transgenic animals can be tailored to show signals in selected cells, tissues, or throughout the body. A tissue-specific promoter restricts fluorescent protein production to particular biological locations, while broader expression produces a more widespread signal. This design connects the visible pattern to the cells or tissues being studied, helping investigators focus on gene activity or cell development in a defined context.
The fluorescent signal provides a way to follow biological events over time in living systems. Investigators can use it to monitor cell development, gene activity, disease progression, or responses to a drug while retaining tissue context. This temporal and spatial information can connect an observed process with the cells or tissues involved, supporting interpretation of disease mechanisms and treatment effects.
Because these animals can be observed while alive, researchers can gather real-time information rather than relying only on a final tissue snapshot. Preserving tissue context shows where a process occurs within the organism, which is important when studying disease progression or treatment responses. This combination helps relate visible biological activity to events that matter in medicine.
Model design begins by selecting the biological location or scope that should be visible. Researchers introduce a fluorescent protein gene and can link it to a tissue-specific promoter when expression must be restricted to particular cells or tissues. They then use appropriate illumination to detect the resulting signal, aligning the model’s construction with the biological process under investigation.
These models support several medical research areas, including cancer, neurobiology, inflammation, and regenerative medicine. Their value depends on the question: investigators may follow disease progression in one study, examine gene activity or cell development in another, and observe treatment responses in a third. The shared advantage is visual access to biological processes within living systems.
During treatment studies, fluorescent animals can reveal how biological processes respond to a drug in a living system. Researchers may track the relevant signal alongside disease progression or gene activity while preserving information about location and tissue context. These observations can improve understanding of treatment effects and contribute to evaluating potential therapies in medically relevant disease models.