Hipsc Cardiomyocytes
Hipsc Cardiomyocytes - Related Videos
05:06
Model of Ischemic Heart Disease and Video-Based Comparison of Cardiomyocyte Contraction Using hiPSC-Derived Cardiomyocytes
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Cited by 4 •
2020
We present a model of ischemic heart disease using cardiomyocytes derived from human induced pluripotent stem cells, together with a method for quantitative evaluation of tissue damage caused by ischemia. This model can provide a useful platform for drug screening and further research on ischemic heart disease.
08:06
Generation of Ventricular-Like HiPSC-Derived Cardiomyocytes and High-Quality Cell Preparations for Calcium Handling Characterization
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Cited by 3 •
2020
Here we describe and validate a method to consistently generate robust human induced pluripotent stem cell-derived cardiomyocytes and characterize their function. These techniques may help in developing mechanistic insight into signaling pathways, provide a platform for large-scale drug screening, and reliably model cardiac diseases.
08:03
Simultaneous Assessment of Cardiomyocyte DNA Synthesis and Ploidy: A Method to Assist Quantification of Cardiomyocyte Regeneration and Turnover
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Cited by 25 •
2016
Quantification of cardiomyocyte turnover is challenging. The protocol described here makes an important contribution to this challenge by enabling accurate and sensitive quantification of neo-cardiomyocyte nuclei generation and nuclei ploidy.
07:50
Isolation and Culture of Neonatal Mouse Cardiomyocytes
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Cited by 187 •
2013
Primary mouse cardiomyocyte cultures are one of the pivotal tools for the investigation of myofibrillar organization and function. The following protocol describes the isolation and culture of primary cardiomyocytes from neonatal mouse hearts. The resulting cardiomyocyte cultures may be subsequently used for a variety of biomechanical, biochemical and cell-biological assays.
07:05
Isolation and Cryopreservation of Neonatal Rat Cardiomyocytes
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Cited by 49 •
2015
The isolation of neonatal rat cardiomyocytes is a time consuming and unpredictable procedure. This study describes methods for cryopreservation and thawing of neonatal rat cardiomyocytes that allows for more efficient use of cells. The thawed NRCMs can be used for various experiments without the need for performing isolations each time.
FAQs
Directed differentiation uses defined developmental signals to move pluripotent cells through mesodermal and cardiac progenitor stages before cardiac lineage formation. This staged progression reflects key transitions in early heart development and helps produce cells with cardiac marker expression, spontaneous beating, electrical activity, and contractility. The resulting sequence provides a framework for evaluating whether differentiation is producing the intended cardiac phenotype.
Several complementary features are important: spontaneous beating demonstrates rhythmic activity, electrical signals indicate excitable cardiac behavior, and contractility reflects mechanical function. Cardiac marker expression provides molecular evidence of lineage identity. Considering these readouts together is more informative than relying on a single observation, because they assess different aspects of the cells' electrical, mechanical, and biological properties.
Although these cells display characteristic cardiac activity and contractility, maturation remains a recognized challenge. Their developmental state can affect how closely experimental findings represent cardiac biology relevant to patients. Consequently, studies should interpret functional observations in the context of maturation and variability rather than assuming that every generated cell population has identical properties or fully reflects mature human heart tissue.
Cells generated from an individual can provide a patient-specific experimental model for studying inherited arrhythmias or cardiomyopathies. Investigators can examine disease-relevant cardiac behavior in a human cellular context and use the same model to explore potential treatments. This approach connects an individual's genetic background with observable cardiac phenotypes, supporting disease analysis that may not be captured by nonpatient-specific systems.
A typical workflow begins with pluripotent human cells, applies defined developmental signals, and guides them through mesodermal and cardiac progenitor stages toward cardiac differentiation. The resulting population is then evaluated for cardiac marker expression, spontaneous beating, electrical activity, and contractility. These observations help determine whether the differentiation process generated cells with the intended cardiac characteristics for downstream study.
They are used for patient-specific disease modeling, drug discovery, and cardiotoxicity testing. Their human cardiac properties allow investigators to examine how candidate treatments or potentially harmful compounds affect electrical activity and contractile behavior in a renewable cellular model. This makes them relevant for evaluating cardiac responses during therapeutic development and for investigating mechanisms associated with inherited cardiac disorders.
hiPSC cardiomyocytes contribute to regenerative research by providing a renewable source of human cardiac cells for investigating possible strategies to address heart injury or dysfunction. Their use remains primarily research-focused because maturation and variability can influence experimental consistency and clinical relevance. These limitations must be considered when interpreting results and when assessing progress toward future clinical translation.