In Vitro Metastasis Model
In Vitro Metastasis Model - Related Videos
08:49
Models of Bone Metastasis
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Cited by 115 •
2012
Animal models are frequently utilized to study cancer metastasis to bone. In this protocol we will describe two common methods of tumor inoculation for bone metastasis studies and briefly describe some of the analyses utilized to monitor and quantify these models.
11:43
Advanced Animal Model of Colorectal Metastasis in Liver: Imaging Techniques and Properties of Metastatic Clones
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Cited by 6 •
2016
The ability of metastatic clones to colonize distant sites depends on their proliferation capacity and/or their ability to survive in the host microenvironment without significant proliferation. Here, we present an animal model that allows quantitative visualization of both types of liver colonization by metastatic clones.
04:58
Brain Metastasis Mouse Model: A Method to Generate Brain Metastasis Mouse Model by Intracarotid Cancer Cell Injection
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2025
This video demonstrates the establishment of a brain metastasis mouse model by intracarotid injection of fluorescently labeled cancer cells. The mouse model can be used to study the effects of novel therapeutics and the underlying physiological mechanisms.
08:40
Quantitative Analysis of Cancer Metastasis using an Avian Embryo Model
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Cited by 90 •
2011
Using quantitative PCR, we demonstrate how the well-established chick CAM model can be used to quantitatively analyze the metastasis of human tumor cells to distant organs.
12:03
In Vivo Model for Testing Effect of Hypoxia on Tumor Metastasis
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Cited by 6 •
2016
This manuscript describes the development of an animal model that allows for the direct testing of the effects of tumor hypoxia on metastasis and the deciphering the mechanisms of its action. Although the experiments described here focus on Ewing sarcoma, a similar approach can be applied to other tumor types.
FAQs
Each format emphasizes different aspects of metastatic behavior. Transwell assays can examine movement across a defined barrier, whereas organotypic cultures provide tissue-like conditions involving extracellular matrix or stromal cells. Microfluidic platforms allow controlled biochemical or physical cues. Comparing these formats helps investigators select a system that isolates migration, invasion, or other selected steps of cancer spread.
These components supply environmental conditions that influence tumor cell behavior outside the body. Extracellular matrix can provide structural context, stromal cells allow tumor–microenvironment interactions to be examined, and tissue-like barriers help model movement through restrictive interfaces. Including or varying these elements enables researchers to study how local surroundings affect metastatic processes under controlled laboratory conditions.
The model can be configured to focus on migration, invasion, intravasation, or colonization rather than treating cancer spread as one undifferentiated event. This separation is useful because each step can be examined in relation to particular matrix, stromal, or barrier conditions. Researchers can therefore compare tumor cell behavior across selected stages and investigate the cues associated with each process.
Researchers place cancer cells in a selected experimental format and expose them to relevant extracellular matrix, stromal cells, or tissue-like barriers. The system is maintained under controlled conditions, then tumor cell behavior is examined in response to defined biochemical or physical cues. The chosen platform determines which metastatic process can be isolated and how the response is measured.
These models are useful when investigators need to examine tumor–microenvironment interactions, evaluate anticancer therapies, or develop approaches intended to better predict treatment responses. Because the systems permit controlled study of selected metastatic behaviors, they can support comparisons between experimental conditions before findings are considered in broader medical research or personalized treatment development.
By exposing tumor cells within a controlled matrix, stromal, barrier, or microfluidic context to biochemical or physical conditions, researchers can measure changes in metastatic behavior. Such measurements may help evaluate how an anticancer therapy affects selected processes such as migration or invasion. The resulting evidence can contribute to more predictive treatment approaches and personalized medicine research.