Intestinal Injury
Intestinal Injury - Related Videos
07:07
Murine Model of Intestinal Ischemia-reperfusion Injury
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Cited by 52 •
2016
Here we describe the detailed procedure of intestinal ischemia-reperfusion in mice which results in reproducible injury without mortality to encourage the standardization of this technique across the field. This model of intestinal ischemia-reperfusion injury can be utilized to study the cellular and molecular mechanisms of injury and regeneration.
10:58
A Protocol for Lentiviral Transduction and Downstream Analysis of Intestinal Organoids
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Cited by 81 •
2015
In this video protocol we give a step by step explanation of lentiviral transduction in organoids of primary intestinal epithelium and of processing and downstream analysis of these cultures by quantitative RT-PCR, RNA-microarray and immunohistochemistry.
04:37
Intestinal Crypts Isolation: A Method to Isolate Whole Crypts from Small Intestine of Murine Model
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2023
In this video, we demonstrate a technique to isolate whole crypts from a mouse’s small intestine. The isolated whole crypts can be used to propagate long-lived and self-renewing 3D organoids in vitro.
17:15
Catheterization of Intestinal Loops in Ruminants
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Cited by 4 •
2009
We describe a novel surgical method for catheterizing 'intestinal loops' within the ileum of sheep. Once animals have recovered from surgery and have cleared antibiotics and analgesics, multiple treatments can be deposited directly in loops via the catheters.
07:28
Controlled Cervical Laceration Injury in Mice
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Cited by 17 •
2013
A novel technique to create a reproducible in vivo model of cervical spinal cord laceration injury in the mouse is described. This technique is based on spine stabilization by fixation of the cervical facets and laceration of the spinal cord using an oscillating blade with an accuracy of ±0.01 mm.
FAQs
Damage to the intestinal epithelium can weaken the barrier that separates gut contents from surrounding tissues. This disruption may promote fluid loss and change how intestinal microbes interact with immune cells. Consequently, the injury can affect local tissue responses as well as the intestine’s ability to maintain normal digestive and absorptive functions.
Inflammation, infection, ischemia, mechanical trauma, and toxic exposure can damage intestinal structure or function through different initiating processes. The resulting pattern may therefore vary in how strongly it affects the epithelial barrier, digestion, absorption, or interactions among microbes and immune cells. Identifying the likely cause helps frame subsequent medical assessment.
The intestine must preserve both functional tissue and a protective epithelial boundary. When injury disrupts these features, digestion and absorption may become less effective, while altered microbial contact with immune cells can influence tissue responses. These linked effects explain why intestinal injury is relevant not only to gastrointestinal function but also to barrier and immune biology.
Assessment focuses on identifying damage to intestinal structure or function and determining its clinical context. This information can support diagnosis and management when intestinal injury is associated with inflammatory bowel disease, intestinal obstruction, or treatment-related toxicity. Evaluation is therefore useful for connecting observed intestinal problems with an underlying disease process or exposure.
Intestinal injury is particularly important in the context of inflammatory bowel disease, intestinal obstruction, and toxicity related to treatment. In these settings, recognizing impaired intestinal structure or function can help inform diagnosis and management. The same assessment may also clarify how disrupted barrier integrity contributes to symptoms or complications associated with the underlying condition.
Research examines epithelial repair, barrier integrity, and tissue regeneration as potential routes to better care. These areas may support development of biomarkers for detecting or characterizing injury, therapies aimed at limiting damage or promoting repair, and recovery strategies that restore intestinal function. The goal is to translate knowledge of tissue responses into improved clinical management.