Iris Vasculature Analysis

Iris vasculature analysis is the study and measurement of blood-vessel patterns within the iris, providing a noninvasive view of ocular microvascular structure relevant to medicine. It typically uses high-resolution eye imaging to visualize vessel geometry, followed by image processing that segments vessels and quantifies features such as density, branching, caliber, and tortuosity. These measurements can support research into ocular development, vascular abnormalities, inflammation, and systemic disease associations. By enabling objective comparison across individuals or over time, iris vasculature analysis may improve understanding of microvascular changes and contribute to earlier assessment of disease-related alterations.

Iris Vasculature Analysis - Related Videos

Research

JoVE Journal - Bioengineering
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Biomolecular Detection employing the Interferometric Reflectance Imaging Sensor (IRIS)

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Cited by 2 •

2011

Quantitative, high-throughput, real-time, and label-free biomolecular detection (DNA, protein, etc.) on SiO2 surfaces can be achieved using a simple interferometric technique which relies on LED illumination, minimal optical components, and a camera. The Interferometric Reflectance Imaging Sensor (IRIS) is inexpensive, simple to use, and amenable to microarray formats.

Research

JoVE Journal - Biology
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A System for Culturing Iris Pigment Epithelial Cells to Study Lens Regeneration in Newt

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Cited by 7 •

2011

In newt, the lens regenerates always from the dorsal iris by transdifferentiation of the iris pigmented epithelial cells (IPEs). Here we describe a procedure to culture dorsal and ventral newt IPE cells and their implantation to the newt eye. The implanted cells are then studied by tissue sectioning and immunohistochemistry.

Research

JoVE Journal - Immunology and Infection

A Method for Labeling Vasculature in Embryonic Mice

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Cited by 14 •

2011

This article describes a method for labeling embryonic skin and thymus blood vessels.

Trypsin Digest Protocol to Analyze the Retinal Vasculature of a Mouse Model

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Cited by 59 •

2013

Trypsin digest is one of the most commonly used methods to analyze retinal vasculature. This manuscript describes the method in detail, including key alterations to optimize the technique and remove the non-vascular tissue while preserving the overall architecture of the vessels.

Whole-mount Immunohistochemical Analysis for Embryonic Limb Skin Vasculature: a Model System to Study Vascular Branching Morphogenesis in Embryo

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Cited by 19 •

2011

We introduce a whole-mount immunohistochemistry and laser scanning confocal microscopy with multiple labelling for analyzing intricate vascular network formation in mouse embryonic limb skin.

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