Lung Graft Preservation
Lung Graft Preservation - Related Videos
08:12
Normothermic Ex Vivo Kidney Perfusion for the Preservation of Kidney Grafts prior to Transplantation
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Cited by 43 •
2015
The severe organ shortage has resulted in increased use of marginal kidney grafts for transplantation. This has triggered interest in alternative storage methods, since marginal grafts especially tolerate cold storage poorly. The technique of normothermic ex vivo kidney perfusion (NEVKP) represents a novel preservation method for kidney grafts prior to transplantation.
02:34
Modeling Preservation Injury in Porcine Donor Kidney: A Procedure to Induce Graft Dysfunction by Subjecting Kidney to Prolonged Cold Ischemia
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2025
In this video, we describe a technique to induce preservation injury via the prolonged cold ischemic storage time in porcine donor kidney.
05:49
Establishment of an Ex Vivo Lung Perfusion Rat Model for Translational Insights in Lung Transplantation
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Cited by 5 •
2023
Here, we describe the necessary steps for establishing a rat EVLP model and show the inflammatory profile associated with the perfused lungs. The aim is to propagate knowledge and experiences about the rat EVLP model, enabling the integral understanding of the biological responses associated with that revolutionary technique.
09:34
Thermal Preconditioning During Ex-vivo Lung Perfusion for the Rehabilitation of Damaged Lung Grafts before Transplantation
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2025
This protocol presents a rat ex-vivo lung perfusion model incorporating a transient exposure to a calibrated thermal stress, promoting endogenous protective mechanisms for the therapeutic reconditioning of damaged lung grafts. The approach is scalable and reproducible and supports the development of non-drug therapies to improve lung transplant success.
07:37
Mouse Models for Graft Arteriosclerosis
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Cited by 17 •
2013
We describe protocols for our mouse graft arteriosclerois (GA) models which involve interposition of a mouse vessel segment into a recipient of the same inbred strain. By backcrossing additional genetic changes into the vessel donor, the model can assess the effect of specific genes on GA.
FAQs
Controlled cooling lowers the graft’s cellular metabolic demand during the interval outside the body. This helps reduce consumption of oxygen and nutrients when normal circulation is absent, limiting conditions that can promote cellular injury. Cooling therefore works as a protective control rather than a replacement for perfusion, supporting tissue viability until transplantation can occur.
Preservation solutions provide a protective chemical environment while the lungs are not connected to the recipient’s circulation. In combination with limited oxygen and nutrient delivery, they help restrain metabolic activity and cellular stress. The effectiveness of this strategy depends on coordinating solution-based protection with temperature control, rather than relying on any single preservation measure.
Edema and ischemic injury are important preservation targets because deterioration during the interval without normal blood flow can compromise graft function. Lung graft preservation addresses these risks by reducing metabolism and maintaining selected supportive conditions. Limiting such injury is clinically relevant because it may lessen the severity of ischemia-reperfusion complications after the graft is implanted.
A preservation workflow begins with donor lung retrieval, followed by controlled cooling and use of a protective preservation solution. The graft then remains under limited oxygen and nutrient support until transplantation, unless ex vivo lung perfusion is added for assessment or rehabilitation. This sequence aims to maintain viability while the organ is transported and prepared for implantation.
Ex vivo lung perfusion circulates a physiologic perfusate through the pulmonary vasculature while the organ remains outside the body. Under monitored conditions, this platform can help assess marginal lungs and may support their rehabilitation before transplantation. Its value is therefore not only preservation, but also providing information about whether an otherwise questionable graft may be usable.
Researchers may apply these strategies when donor lungs require protection between retrieval and transplantation or when an organ’s suitability needs closer evaluation. Ex vivo perfusion is especially relevant to marginal organs, whereas cooling and solution-based methods provide the underlying preservation framework. Together, they can expand the usable donor pool, improve graft assessment, and potentially reduce post-transplant ischemia-reperfusion complications.