Mechanical De-epithelialization

Mechanical de-epithelialization is a medical technique that removes an epithelial layer through physical manipulation, exposing the underlying tissue for treatment, healing, or reconstruction. Clinicians typically use controlled scraping, abrasion, or related instruments to detach epithelial cells while limiting injury to deeper structures; the extent and method depend on the target tissue and clinical objective. This approach can improve access to the tissue surface, support procedures that require epithelial removal, and help researchers study barrier repair and tissue regeneration. Its effectiveness and safety depend on precise technique, appropriate tissue handling, and careful management of the exposed area during recovery.

Mechanical De-epithelialization - Related Videos

Research

JoVE Journal - Developmental Biology
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Isolation of Mouse Endometrial Epithelial and Stromal Cells for In Vitro Decidualization

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Cited by 86 •

2017

This study presents a standardized and validated method for the isolation and culture of primary mouse endometrial stromal and epithelial cells, which can be used in a coculture system to study in vitro decidualization.

Research

JoVE Journal - Medicine

Development of Human Renal Tubular Epithelial Cell Primary Cultures in Monolayers and Three-Dimensional Conditions

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2025

Here, we describe the protocols for obtaining primary cultures of human renal tubular epithelial cells (HRTEC) from the kidney cortex to develop monolayers and three-dimensional cultures on an extracellular matrix (3D-HRTEC).

In Vitro Assay of Bacterial Adhesion onto Mammalian Epithelial Cells

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Cited by 81 •

2011

This protocol is a simple bacterial adhesion assay consisting in counting the numbers of bacterial colony forming units that are adhered onto cultured cells. The assay is robust, independent of the adhesin studied, and numerous variations are used in most laboratories working on bacterial pathogenesis.

Live Cell Imaging during Mechanical Stretch

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Cited by 12 •

2015

A novel imaging protocol was developed using a custom motor-driven mechanical actuator to allow the measurement of real time responses to mechanical strain in live cells. Relevant to mechanobiology, the system can apply strains up to 20% while allowing near real-time imaging with confocal or atomic force microscopy.

Imaging Ca2+ Responses During Shigella Infection of Epithelial Cells

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Cited by 4 •

2018

Here, we present protocols to visualize calcium (Ca2+) responses elicited by HeLa cells infected by Shigella. By optimizing the parameters of bacterial infection and imaging with Ca2+ fluorescent probes, atypical global and local Ca2+ signals induced by bacteria over a large range of infection kinetics are characterized.

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