Mouse Islets
Mouse Islets - Related Videos
03:33
Grafting Pancreatic Islets: A Method to Implant Pancreatic Islets into the Anterior Chamber of the Mouse Eye for In Vivo Imaging
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2023
This video describes the protocol for grafting human pancreatic islets into the anterior chamber of the mouse’s eye to monitor revascularization of human islet grafts in vivo. It also helps to understand the contribution of recipient versus donor cells in promoting the encapsulation and vascularization of the graft.
07:04
Effective Isolation of Functional Islets from Neonatal Mouse Pancreas
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Cited by 19 •
2017
We describe a protocol herein for isolating intact islets from neonatal mice. Pancreata were partially digested with collagenase, followed by washing and hand picking. 20 - 80 islet clusters can be obtained per pancreas from newly born mice, which are suitable for several islet studies.
07:21
Pancreatic Islets Isolation through Collagenase Perfusion: An Enzymatic Method to Isolate Pancreatic Islet Cells
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2023
Pancreatic islets, also called the Islets of Langerhans, are a cluster of endocrine cells which produces hormones for glucose regulation and other important biological functions. This protocol explains a method for isolating pancreatic islets by first perfusing collagenase via Ampulla of Vater to digest the exocrine pancreatic tissue and a simplified density gradient centrifugation to purify the islets from mice.
03:48
Murine Model of Islet Transplantation: A Surgical Method to Infuse Pancreatic Islets in Portal Vein and Prevention of Postoperative Bleeding
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2025
In this video, we describe a procedure to infuse pancreatic islet cells into the hepatic portal vein, followed by application of gel foam to successfully prevent bleeding. Postoperative bleeding is a common cause of high mortality during islet transplantation.
12:33
A Method for Mouse Pancreatic Islet Isolation and Intracellular cAMP Determination
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Cited by 54 •
2014
Assaying in vitro β-cell function using isolated mouse islets of Langerhans is an important component in the study of diabetes pathophysiology and therapeutics. While many downstream applications are available, this protocol specifically describes the measurement of intracellular cyclic adenosine monophosphate (cAMP) as an essential parameter determining β-cell function.
FAQs
Beta cells respond to rising glucose by increasing insulin release, whereas alpha cells produce glucagon when glucose declines. Studying these responses together helps investigators examine coordinated hormonal control rather than analyzing either cell type in isolation. This cellular combination is especially useful for exploring how glucose regulation becomes disrupted during diabetes and other metabolic disorders.
Keeping several endocrine cell types together preserves opportunities to examine communication within the islet. Interactions among beta cells, alpha cells, and other endocrine populations can influence hormone secretion and glucose regulation. Consequently, mouse islets may reveal regulatory changes that isolated cell preparations could miss, making them valuable for investigating tissue-level mechanisms of metabolic balance.
Comparing responses to rising and falling glucose is informative because the two conditions engage different hormonal outputs. Increased glucose tests insulin-related beta-cell activity, while reduced glucose provides a context for examining glucagon secretion from alpha cells. This contrast helps researchers evaluate whether an islet maintains appropriate, coordinated responses across changing metabolic conditions.
Isolated mouse islets provide a focused system for examining beta-cell function, hormone secretion, and changes associated with pancreatic injury or diabetes. Because the preparation retains interactions among multiple endocrine cell types, investigators can connect altered cellular communication with impaired glucose regulation. These observations help clarify mechanisms underlying metabolic disease without relying solely on whole-animal measurements.
Mouse islets support drug screening by providing a model in which investigators can examine effects on beta-cell function and hormone secretion. They also contribute to transplantation studies, where preserved endocrine organization offers a way to evaluate islet-related behavior in a therapeutic research context. These applications connect cellular findings with potential strategies for addressing diabetes.
In medicine, mouse islets serve as an experimental bridge between cellular mechanisms and diseases involving glucose control. Their use can support studies of diabetes, metabolic disease, pancreatic injury, therapeutic screening, and transplantation. Results can illuminate how endocrine-cell communication contributes to impaired regulation, while also providing a model for testing research questions before broader biological evaluation.