Porcine Corneal Culture
Porcine Corneal Culture - Related Videos
04:11
Laser-assisted Selective Ablation of Porcine Corneal Endothelial Cells: A Technique to Induce Focused Porcine Corneal Endothelial Injury for Ex Vivo Studies
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2025
This video demonstrates a non-invasive laser-assisted procedure to selectively damage the corneal endothelial cells in the porcine eye. The developed ex vivo model of endothelial cell damage can be used to study diseases of the corneal endothelium.
08:36
A Porcine Corneal Endothelial Organ Culture Model Using Split Corneal Buttons
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Cited by 4 •
2019
Here, a step-by-step protocol for the preparation and cultivation of porcine split corneal buttons is presented. As this organo-typically cultivated organ culture model shows cell death rates within 15 days, comparable to human donor corneas, it represents the first model allowing long-term cultivation of non-human corneas without adding toxic dextran.
05:00
Porcine Ex vivo Cornea Model of Bacterial Keratitis: A Technique to Establish Bacterial Infection in Corneal Epithelial Cells
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2025
This video describes a protocol to set up an ex vivo model for bacterial keratitis using a corneoscleral button dissected from the porcine eye. Inoculated bacteria traverse the corneal epithelial incisions to invade the underlying stroma and establish an infection within the corneal tissues that mimic the in vivo infection.
08:17
Organotypic Culture of Full-thickness Adult Porcine Retina
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Cited by 19 •
2011
Here we describe a cost-effective technique for organotypic culture of adult porcine retina for seven days. Briefly, a sterile filter paper was used to lift the neural retina off from the RPE and place photoreceptor side up on an insert raised by a custom-made stand.
03:33
Generating a 3D Co-Culture Model of Human Corneal Fibroblasts and Neurons
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2025
This video demonstrates a technique to generate a three-dimensional co-culture model system of primary human corneal fibroblasts and differentiated neuronal cells. This co-culture system can be used to study the stromal-nerve interactions.
FAQs
Organ culture retains aspects of the cornea’s native structure, allowing investigators to examine tissue-level behavior and interactions. Isolated-cell systems instead focus on cellular responses under controlled conditions, which can simplify studies of epithelial repair or inflammatory reactions. Choosing between them depends on whether the research question requires preserved architecture or more direct analysis of individual cell populations.
These conditions help preserve cellular viability during laboratory maintenance. Appropriate gas exchange supports the cultured tissue environment, while osmotic regulation helps prevent damaging fluid shifts across cells. Temperature control further stabilizes biological activity. If these variables are poorly regulated, tissue or isolated cells may lose viability, reducing the reliability of measurements involving repair, infection, or treatment responses.
Porcine corneas share key anatomical and physiological features with human corneas, making them biologically relevant for ocular medicine research. This similarity supports evaluation of how tissues respond to therapeutic compounds, contact lenses, or surgical materials before clinical research. The model does not replace human studies, but it can provide evidence in a system that better reflects ocular tissue behavior than a purely artificial model.
A typical workflow begins with either explanted corneal tissue or isolated corneal cells, followed by placement in sterile nutrient media. The culture is then maintained with regulated temperature, gas exchange, and osmotic conditions. The selected format should match the study objective: preserved tissue for structural responses, or isolated cells for more focused cellular investigations. Sterile handling is essential throughout maintenance.
The model can support studies of epithelial repair, infection, inflammation, drug penetration, and responses to contact lenses or surgical materials. These applications allow investigators to examine several clinically relevant processes using related tissue or cell formats. The resulting observations can help characterize treatment effects and material interactions before advancing to animal studies or clinical research.
Porcine corneal culture provides a controlled laboratory setting for examining ocular treatments and tissue responses before clinical research. It can help investigators study drug penetration, repair, infection, inflammation, or material compatibility without relying exclusively on whole-animal experiments. Because the tissue retains relevant porcine anatomical and physiological features, findings may offer useful preclinical context while remaining distinct from clinical evidence.