Unilateral Contusion Model
Unilateral Contusion Model - Related Videos
07:28
A Contusive Model of Unilateral Cervical Spinal Cord Injury Using the Infinite Horizon Impactor
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Cited by 46 •
2012
A reliable and repeatable way to produce a cervical unilateral spinal cord injury using the Infinite Horizon impactor is described. The method takes advantage of a custom designed frame and clamp to stabilize the spine. The standardized procedure and biomechanical injury parameters result in sufficient and sustained injuries.
10:00
A Contusion Model of Severe Spinal Cord Injury in Rats
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Cited by 41 •
2013
A contusion model of severe spinal cord injury is described. Detailed pre-operative, operative and post-operative steps are described to obtain a consistent model.
14:05
A Murine Model of Irreversible and Reversible Unilateral Ureteric Obstruction
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Cited by 47 •
2014
The murine model of irreversible unilateral ureteric obstruction (UUO) is presented together with the model of reversible UUO in which the ureteric obstruction is relieved by anastomosis of the severed ureter into the bladder. These models enable the study of renal inflammation and scarring as well as tissue remodeling.
02:13
Inducing a Spinal Cord Contusion in a Mouse Model Using a Spinal Cord Injury Coaxial Platform
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2025
Source: Elzat, E. Y. Y., et al., Establishing a Mouse Contusion Spinal Cord Injury Model Based on a Minimally Invasive Technique. J. Vis. Exp. (2022).This video demonstrates the method for inducing a contusion injury at the T9 level spinal cord in a mouse model, using a spinal cord injury coaxial platform. A weight is dropped on the T9 level spinal cord that creates a contusion that immediately causes tissue discoloration and swelling.
10:09
Development of a Unilaterally-lesioned 6-OHDA Mouse Model of Parkinson's Disease
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Cited by 121 •
2012
A protocol for performing unilateral 6-OHDA lesions of the medial forebrain bundle in mice is described. This method has a low mortality rate (13.3 %) with 89% of the surviving animals showing >95% loss of striatal dopamine and 90.63±-4.02 % ipsiversive rotational bias towards the side of the lesion.
FAQs
The impact applies controlled mechanical force to one tissue region, disrupting cells and small blood vessels. This local damage initiates inflammation, promotes fluid accumulation as edema, and eventually engages tissue remodeling. Because the injury is produced under controlled conditions, researchers can examine how these linked responses develop at the affected site rather than interpreting damage from an uncontrolled traumatic event.
The unaffected side can serve as an internal comparison for the injured tissue. This design helps researchers distinguish changes associated with the contusion from features that are naturally present in the same experimental system. Comparing the two sides supports clearer assessment of localized damage, biological markers, and recovery-related changes without relying only on comparisons between separate subjects or specimens.
Progression can be followed through the sequence of localized cellular and vascular disruption, inflammation, edema, and tissue remodeling. These processes represent changing stages of the tissue response rather than a single endpoint. Examining them helps researchers characterize how injury develops and recovers, while also identifying biological markers that may reflect damage or repair at the affected location.
Calibration establishes a controlled injury stimulus, making the resulting tissue response more suitable for comparison across experimental conditions. The impact must be applied to the intended side and tissue or organ so that observed differences can be interpreted in relation to the induced contusion. This control is important when evaluating injury progression or comparing treatment effects.
A study first identifies the tissue or organ and the side designated for injury. Researchers then apply a calibrated impact to that side, leaving the opposite side unaffected for comparison. Subsequent assessment focuses on localized damage, inflammation, edema, remodeling, or biological markers. The same comparison can also be used to examine whether an intervention changes injury or recovery.
Researchers may choose it when they need to test whether a treatment reduces localized damage or improves repair after blunt-force injury. The side-specific design allows treated injury responses to be interpreted alongside unaffected tissue within the same experimental framework. Outcomes may include changes in injury progression, biological markers, inflammation-related effects, edema, or tissue remodeling.