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1Experimental and Clinical Research Center (ECRC),Charité Medical Faculty and Max-Delbrück Center for Molecular Medicine (MDC), 2Medical Department, Division of Cardiology, Campus Virchow-Klinikum,Charité – Universitätsmedizin Berlin, 3Medical Department, Division of Cardiology and Angiology, Campus Mitte,Charité – Universitätsmedizin Berlin
Chapters
- 00:05Title
- 01:37Animal Anesthesia and Organ Harvesting
- 03:00Preparation of Heart and Langendorff Perfusion
- 04:03Dissociation of Cardiomyocytes
- 06:30Preparation of Cardiomyocytes for Cellular Electrophysiology, Biochemical of IF Studies
- 08:53Cellular Electrophysiology
- 10:33Results: Isolation and Kv Channel Recordings in Murine Atrial and Ventricular Cardiomyocytes
- 11:04Conclusion
Kv channel dysfunction is associated with cardiac arrhythmias. In order to study the molecular mechanisms that lead to such arrhythmias we utilize a systematic protocol for isolation of atrial and ventricular cardiomyocytes from Kv channel ancillary subunit knockout mice. Isolated cardiomyocytes can then immediately be used for cellular electrophysiological studies, biochemical or immunofluorescence (IF) assays.
