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Isolation and Kv Channel Recordings in Murine Atrial and Ventricular Cardiomyocytes
 

Isolation and Kv Channel Recordings in Murine Atrial and Ventricular Cardiomyocytes

Article doi: 10.3791/50145
March 12th, 2013

Summary March 12th, 2013

Kv channel dysfunction is associated with cardiac arrhythmias. In order to study the molecular mechanisms that lead to such arrhythmias we utilize a systematic protocol for isolation of atrial and ventricular cardiomyocytes from Kv channel ancillary subunit knockout mice. Isolated cardiomyocytes can then immediately be used for cellular electrophysiological studies, biochemical or immunofluorescence (IF) assays.

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