Adult Hippocampal Neurogenesis
Adult Hippocampal Neurogenesis - Related Videos
04:18
An Immunofluorescence Staining Technique to Detect Adult Hippocampal Neurogenesis
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2025
The video illustrates an immunofluorescence staining method used to examine neurogenesis in the adult hippocampus. Initially, a mouse brain section undergoes treatment with primary antibodies directed at marker proteins associated with neurogenesis in neuronal progenitor cells. Next, it is labeled with secondary antibodies tagged with fluorophores. The brain section is then observed under a confocal microscope to distinguish various subtypes of progenitor cells, based on the expression patterns...
09:38
Heat-Induced Antigen Retrieval: An Effective Method to Detect and Identify Progenitor Cell Types during Adult Hippocampal Neurogenesis
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Cited by 23 •
2013
During adult hippocampal neurogenesis, a distinct set of genetic markers are expressed when a quiescent neural stem cell sequentially progresses and develops into a functionally integrated neuron in the circuit. Using heat-induced antigen retrieval, progenitor cell types that are otherwise difficult to detect are identified with improved effectiveness.
09:24
Immunohistochemistry and Multiple Labeling with Antibodies from the Same Host Species to Study Adult Hippocampal Neurogenesis
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Cited by 20 •
2015
This video article illustrates a comprehensive protocol to detect and quantify all stages of adult hippocampal neurogenesis within the same tissue section. We elaborated a method to overcome the limitations of indirect multiple immunofluorescence that arise when suitable antibodies from different host species are unavailable.
14:58
Methods for the Modulation and Analysis of NF-κB-dependent Adult Neurogenesis
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Cited by 1 •
2014
Methods for the manipulation and analysis of NF-κB-dependent adult hippocampal neurogenesis are described. A detailed protocol is presented for a dentate gyrus-dependent behavioral test (termed the spatial pattern separation-Barnes maze) for the investigation of cognitive outcome in mice. This technique should also help enable investigations in other experimental settings.
11:45
Functional Interrogation of Adult Hypothalamic Neurogenesis with Focal Radiological Inhibition
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Cited by 8 •
2013
The function of adult-born mammalian neurons remains an active area of investigation. Ionizing radiation inhibits the birth of new neurons. Using computer tomography-guided focal irradiation (CFIR), three-dimensional anatomical targeting of specific neural progenitor populations can now be used to assess the functional role of adult neurogenesis.
FAQs
Within the dentate gyrus, the process follows a sequence rather than a single cellular event: neural stem and progenitor cells proliferate, generate immature neurons, and then pass through differentiation and maturation. The resulting cells must also integrate into existing neural circuits. Considering each stage separately helps researchers determine where changes in neurogenesis may affect hippocampal function.
Aging, stress, and physical activity are important variables because they can influence the process, although the overview does not specify the direction or magnitude of every effect. Studying these factors allows neuroscience researchers to connect environmental or physiological conditions with changes in new-neuron generation and maturation, potentially clarifying why hippocampal plasticity varies across contexts.
The maturation and circuit-integration stages matter because newly generated cells are not simply counted; they become part of an established hippocampal network. This distinction connects cellular events with broader functions associated with the hippocampus, including learning, memory, and emotional regulation. It also makes adult hippocampal neurogenesis a useful model for examining how structural change can accompany functional plasticity.
At a conceptual level, an investigation can be organized around the dentate gyrus and the successive stages identified in the overview: proliferation, production of immature neurons, differentiation, maturation, and circuit integration. Researchers can then examine how those cellular changes relate to learning, memory, or emotional regulation. This framework links cellular observations to neuroscience questions without reducing the process to cell production alone.
Its relevance comes from the combination of new-neuron generation and incorporation into existing circuits. That combination offers a model for asking how nervous tissue might change, adapt, or potentially support repair after development. The topic therefore helps researchers investigate repair-related mechanisms while also connecting them to disorders involving neuropsychiatric and neurodegenerative processes.
Adult hippocampal neurogenesis provides a way to connect cellular plasticity with learning, memory, and emotional regulation. Researchers can also consider how age, stress, and physical activity relate to these cellular changes, rather than studying hippocampal function independently from its biological conditions. This broader perspective supports investigations of brain plasticity and may inform research on neuropsychiatric and neurodegenerative disorders.