Cilium Marker Arl13b
Cilium Marker Arl13b - Related Videos
08:14
Using Primary Neurosphere Cultures to Study Primary Cilia
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Cited by 3 •
2017
The primary cilium is fundamentally important in neural progenitor cell proliferation, neuronal differentiation, and adult neuronal function. Here, we describe a method to study ciliogenesis and the trafficking of signaling proteins to cilia in neural stem/progenitor cells and differentiated neurons using primary neurosphere cultures.
07:53
Application of High-speed Super-resolution SPEED Microscopy in Live Primary Cilium
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Cited by 4 •
2018
Recently we mapped the three-dimensional (3D) spatial locations of transport routes for various proteins translocating inside primary cilia in live cells. Here this paper details the experimental setup, the process of biological samples and the data analyses for the 3D super-resolution fluorescence imaging approach newly applied in live primary cilia.
09:53
Volumetric Imaging and Analysis of Primary Cilia in Musculoskeletal Tissue using the ARL13B-CENTRIN-2 Mouse Model
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Cited by 1 •
2025
This method describes the exploitation of the endogenous signal in an ARL13B-CENTRIN-2 transgenic mouse model to image primary cilia in situ, in three dimensions across large areas of mineralized tissue, from tissue collection to analysis of cilia organization. This protocol may also be applied to other types of tissues.
09:38
Multiplexed Immunofluorescence Assay for Spatial Assessment of Senescence Markers in vivo
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2026
Multiplexed immunofluorescence enables sequential labeling of multiple antibody markers within a single tissue section to support spatial analysis of tissue architecture and cell populations. Here, a workflow for multiplex detection of senescence-associated, structural, and immune markers is presented, including manual and automated slide preparation, iterative imaging, and single-cell analysis using digital pathology software.
12:03
The Production of C. elegans Transgenes via Recombineering with the galK Selectable Marker
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Cited by 4 •
2011
The ability to produce transgenes for Caenorhabditis elegans using genomic DNA carried by fosmids is particularly attractive as all of the native regulatory elements are retained. Described is a simple and robust procedure for the production of transgenes via recombineering with the galK selectable marker.
FAQs
Arl13b is enriched in the ciliary membrane rather than distributed uniformly throughout the cell. This concentrated localization produces a spatial signal that can be evaluated against surrounding cellular structures during imaging. In neural cells and brain tissue, that distinction helps researchers identify cilia more specifically and assess whether labeled projections occupy expected cellular locations.
Membrane enrichment gives Arl13b labeling a useful anatomical reference for identifying the ciliary compartment. Because the signal is associated with the ciliary membrane, researchers can examine the shape and extent of individual labeled projections instead of relying only on general cellular staining. This supports more focused analysis of cilium structure in neuroscience experiments.
Arl13b labeling can support quantitative assessment of cilium number, length, and distribution. These measurements provide different views of ciliary organization: number indicates how frequently cilia are observed, length describes their structural extent, and distribution shows where they occur among neural cells or within brain tissue. Together, the measures can reveal altered ciliary patterns.
Detection is performed through immunofluorescence, which makes Arl13b localization visible for microscopic analysis. Researchers examine the resulting labeling to identify ciliary structures and compare their occurrence across neural cells or tissue regions. The approach is therefore suited to both recognizing individual labeled projections and collecting measurements such as cilium number, length, and distribution.
These measurements are relevant when investigators study neuronal development, cilium-dependent signaling, or conditions associated with abnormal nervous-system development. Comparing number, length, or distribution across neural cells or brain tissue can show how ciliary organization differs between experimental contexts. The resulting structural data help connect visible cilium patterns with broader questions about nervous-system biology.
Arl13b labeling can support studies linking ciliary structure with neuronal development and signaling. In brain tissue, researchers can examine where labeled cilia occur and whether their structural characteristics change in disorders involving abnormal nervous-system development. These observations provide a way to investigate cilium-related alterations while keeping the analysis focused on measurable features of neural tissue.