Using Primary Neurosphere Cultures to Study Primary Cilia

The overall goal of this procedure is to utilize neurosphere based culturing methods for primary neural stem and progenitor cells to study primary cilium biogenesis and trafficking. This method can help answer key questions in the primary cilia field such as cilia biogenesis and trafficking of cilia molecules to the primary cilia. In primary neural stem cells, neural progenitors and neurons.

The main advantage of this technique is that we can use the primary cell criteria of the neural stem cells, neural progenitors and neurons to analyze biogenesis and ciliary pathways in the context of the primary cilium. Generally, individuals new to this method will struggle because culturing neural stem cells is technically challenging for beginners. We'll show a demonstration of this method is critical, as dissection of the lateral ventricle and steps such as culturing neurospheres and quantification of primary cilia are easily learned by observing the critical steps.