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Application of High-speed Super-resolution SPEED Microscopy in Live Primary Cilium
Application of High-speed Super-resolution SPEED Microscopy in Live Primary Cilium
JoVE Journal
Biology
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JoVE Journal Biology
Application of High-speed Super-resolution SPEED Microscopy in Live Primary Cilium

Application of High-speed Super-resolution SPEED Microscopy in Live Primary Cilium

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07:53 min

January 16, 2018

DOI:

07:53 min
January 16, 2018

8334 Views
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Summary

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Recently we mapped the three-dimensional (3D) spatial locations of transport routes for various proteins translocating inside primary cilia in live cells. Here this paper details the experimental setup, the process of biological samples and the data analyses for the 3D super-resolution fluorescence imaging approach newly applied in live primary cilia.

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