Endothelial Network
Endothelial Network - Related Videos
![Development and Characterization of <em>In Vitro</em> Microvessel Network and Quantitative Measurements of Endothelial [Ca<sup>2+</sup>]<sub>i </sub>and Nitric Oxide Production](/_next/image?url=https%3A%2F%2Fcloudfront.jove.com%2FCDNSource%2Fteasers%2F54014.jpg%3Fv%3D2026-09-01%252006%253A38%253A51&w=750&q=75)
09:39
Development and Characterization of In Vitro Microvessel Network and Quantitative Measurements of Endothelial [Ca2+]i and Nitric Oxide Production
0 Views •
Cited by 7 •
2016
Primary human umbilical vein endothelial cells (HUVECs) were grown to confluence within a microfluidic network device. The endothelial cell junction and F-actin distributions were illustrated and the changes in intracellular calcium concentration and nitric oxide production in response to adenosine triphosphate (ATP) were quantified in real-time at individual cell levels.
06:07
Direct Bioprinting of 3D Multicellular Breast Spheroids onto Endothelial Networks
0 Views •
Cited by 7 •
2020
The goal of this protocol is to directly bioprint breast epithelial cells as multicellular spheroids onto pre-formed endothelial networks to rapidly create 3D breast-endothelial co-culture models which can be used for drug screening studies.
01:44
Establishing a Co-Culture of Interneurons with Periventricular Endothelial Cells
0 Views •
2025
This video demonstrates a technique for establishing a co-culture of interneurons with perivascular endothelial cells. The cells are added to a one-well insert placed inside a culture dish. After the cells adhere to the dish, the insert is removed, allowing the cells to proliferate and interact and form a co-culture.
07:36
Isolation of Mouse Coronary Endothelial Cells
0 Views •
Cited by 18 •
2016
This protocol is prepared to share our method of isolating mouse coronary endothelial cells for the purpose of imaging or to conduct molecular biological experiments.
05:41
Non-invasive Assessment of Microvascular and Endothelial Function
0 Views •
Cited by 12 •
2013
Capillaroscopy is a non-invasive, relatively inexpensive methodology for directly visualizing the microcirculation. The forearm blood flow technique provides accepted non-invasive measures of endothelial function.
FAQs
Tight and adherens junctions connect neighboring endothelial cells and help regulate the interface between blood and nervous tissue. Their organization affects how selectively substances move across the blood-brain barrier, rather than allowing unrestricted exchange. Examining these junctions therefore helps researchers evaluate how effectively an endothelial network supports controlled communication between circulation and surrounding neural tissues.
Signals that guide angiogenesis influence how endothelial cells organize into developing or changing vascular arrangements. These signals are important because vessel formation and remodeling determine how the network adapts within nervous tissue. Studying the response to such signals can reveal how vascular structures develop, change, and potentially contribute to altered neurovascular organization.
The network provides a vascular component for examining coordination among blood vessels, neurons, and glial cells. This relationship is relevant because the neurovascular unit supports brain function through interactions among these cell types. Endothelial network studies can therefore connect barrier regulation and vascular behavior with broader questions about how neural tissue is maintained.
A useful model can focus on endothelial cell connectivity, junctional organization, selective transport across the blood-brain barrier, and responses involved in angiogenesis or vessel remodeling. Including these features allows researchers to examine both barrier behavior and vascular change. The resulting system can support investigation of how endothelial properties relate to neural tissue and disease-associated processes.
These models are relevant to research on stroke and neurodegenerative disease, as well as vascular development. They give investigators a way to examine endothelial behavior and its relationship to the surrounding nervous system in defined experimental settings. Comparing network properties across these contexts can help organize studies of vascular dysfunction, remodeling, and brain-related pathology.
Because endothelial cells regulate selective transport across the blood-brain barrier, their network provides a system for studying how therapeutic substances may interact with that barrier. Models can help researchers examine transport-related behavior while considering the surrounding neurovascular context. This makes the approach relevant to developing strategies intended to improve delivery of therapeutics to brain tissue.