Waiting
Login processing...

Trial ends in Request Full Access Tell Your Colleague About Jove
JoVE Journal
Biology

A subscription to JoVE is required to view this content.

Development and Characterization of In Vitro Microvessel Network and Quantitative Measurements of Endothelial [Ca2+]i and Nitric Oxide Production
 

Development and Characterization of In Vitro Microvessel Network and Quantitative Measurements of Endothelial [Ca2+]i and Nitric Oxide Production

Article DOI: 10.3791/54014 09:39 min May 19th, 2016
May 19th, 2016

Chapters

Summary

Primary human umbilical vein endothelial cells (HUVECs) were grown to confluence within a microfluidic network device. The endothelial cell junction and F-actin distributions were illustrated and the changes in intracellular calcium concentration and nitric oxide production in response to adenosine triphosphate (ATP) were quantified in real-time at individual cell levels.

Transcript

Tags

Development Characterization In Vitro Microvessel Network Quantitative Measurements Endothelial [Ca2+]i Nitric Oxide Production Microfluidic Device Shear Flow Agonist Induced Changes Calcium Versatile Design Microchannels Vasculature Cell Culture Environment Photolithography Master Mold Soft Lithography PDMS Microchannel Devices Fibronectin PBS
Read Article

Get cutting-edge science videos from JoVE sent straight to your inbox every month.

Waiting X
Simple Hit Counter