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JoVE Journal Neuroscience
Transplantation of Human Stem Cell-Derived GABAergic Neurons into the Early Postnatal Mouse Hippocampus to Mitigate Neurodevelopmental Disorders
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Transplantation of Human Stem Cell-Derived GABAergic Neurons into the Early Postnatal Mouse Hippocampus to Mitigate Neurodevelopmental Disorders

Article DOI: 10.3791/64272-v • 05:00 min • November 11th, 2022
November 11th, 2022 •
2, 1, 1, 3, 2, 1
1Cellular Neurophysiology and Epilepsy Group, Epilepsy Centre, Department of Clinical Sciences, Lund University Hospital, 2Experimental Epilepsy Group, Epilepsy Centre, Department of Clinical Sciences, Lund University Hospital, 3Molecular Neurophysiology and Epilepsy Group, Epilepsy Center, Department of Clinical Sciences, Lund University Hospital

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Summary
Automatic Translation

Transplantation of human pluripotent stem cell-derived GABAergic neurons generated by neuronal programming could be a potential treatment approach for neurodevelopmental disorders. This protocol describes the generation and transplantation of human stem cell-derived GABAergic neuronal precursors into the brains of neonatal mice, allowing long-term investigation of grafted neurons and evaluation of their therapeutic potential.

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Human Stem Cell GABAergic Neurons Transplantation Postnatal Mouse Hippocampus Neurodevelopmental Disorders Cellular Identity Integration Functionality Interneurons Developing Brains Protocol Generation Survival Maturation Therapeutic Potential Cell Therapy Developmental Disorders Interneuron Precursors Cell Culture Medium DPBS Cell Detachment Solution N2 Medium Single-cell Suspension Centrifuge
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