In Vivo Recording
In Vivo Recording - Related Videos
12:32
Retrograde Fluorescent Labeling Allows for Targeted Extracellular Single-unit Recording from Identified Neurons In vivo
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Cited by 13 •
2013
Retrograde transport of fluorescent dye labels a sub-population of neurons based on anatomical projection. Labeled axons can be visually targeted in vivo, permitting extracellular recording from identified axons. This technique facilitates recording when neurons cannot be labeled through genetic manipulation or are difficult to isolate using 'blind' in vivo approaches.
11:00
Single-unit In vivo Recordings from the Optic Chiasm of Rat
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Cited by 3 •
2010
Retinal ganglion cells transmit visual information from the eye to the brain with sequences of action potentials. Here, we demonstrate how to record the action potentials of single ganglion cells in vivo from anesthetized rats.
14:19
Micro-drive Array for Chronic in vivo Recording: Tetrode Assembly
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Cited by 111 •
2009
In this protocol we demonstrate how to fabricate and condition tetrodes for use with a micro-drive array, which was designed for chronic electrophysiological recordings in rats. In addition, we illustrate the final stages of micro-drive array construction, which includes installing ground wires and a protective cone.
10:41
A Wireless, Bidirectional Interface for In Vivo Recording and Stimulation of Neural Activity in Freely Behaving Rats
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Cited by 12 •
2017
A wireless, bidirectional system for multi-channel neural recordings and stimulation in freely behaving rats is introduced. The system is light and compact, thus having minimal impact on the animal´s behavioral repertoire. Moreover, this bidirectional system provides a sophisticated tool to assess causal relationships between brain activation patterns and behavior.
02:36
In Vivo Recording of Intracellular Signals from a Single Neuron
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2025
This video demonstrates the recording of electrical signals from single brain neurons using a fine-tipped glass micropipette and a specialized setup. The micropipette is carefully inserted into the rat's brain and is buzzed to penetrate the neuron to record internal neuronal electrical signals.
FAQs
The approach can detect changes in membrane voltage, action potentials, or intracellular calcium. These signal types provide different views of neural activity, from electrical changes associated with neuronal signaling to calcium changes that indicate activity within cells. Selecting among them helps researchers relate cellular responses to sensory inputs, experimental tasks, circuit function, or behavior.
These approaches access neural activity through different measurement interfaces. Implanted electrodes record within the organism, surface sensors detect activity from outside the tissue, and genetically encoded optical indicators report changes such as intracellular calcium. The choice determines which signals can be measured and how directly recordings can connect cellular activity with circuit and behavioral responses.
Measurements made under living physiological conditions preserve interactions among neurons, circuits, and behavior. This context allows neural responses to be examined during sensory input or experimental tasks rather than as isolated cellular events. Consequently, researchers can study how activity is organized across levels of the nervous system and relate neural dynamics to observable behavior.
Researchers select the recording approach according to the neural signal and experimental relationship they need to examine. Implanted electrodes, surface sensors, or genetically encoded optical indicators can be used to detect electrical or calcium-related activity while an organism receives sensory input or performs a task. This alignment helps connect measured signals with circuit function and behavior.
The method supports investigations of perception, learning, movement, and disease mechanisms. By measuring activity during relevant sensory experiences or experimental tasks, researchers can examine how neural signals relate to these functions. Its value lies in linking cellular and circuit-level dynamics with behavior, rather than studying neural activity independently from the organism’s actions or responses.
Because recordings can be collected in living organisms over time, they support evaluation of neural dynamics and interventions across multiple stages of an investigation. Repeated measurements can help researchers examine how activity changes in relation to an intervention, while continued observation preserves the connection between evolving neural signals, circuit function, and behavior.