Neural Tube Slice
Neural Tube Slice - Related Videos
12:48
Isolation and Culture of Neural Crest Cells from Embryonic Murine Neural Tube
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Cited by 26 •
2012
Isolation of embryonic neural crest from the neural tube facilitates the use of in vitro methods for studying migration, self-renewal, and multipotency of neural crest.
07:37
Neural Tube Closure in Mouse Whole Embryo Culture
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Cited by 22 •
2011
A method allowing for direct pharmacological manipulation of mouse embryos during neurulation that bypasses maternal metabolism is described. The technique can be adapted to study different aspects of neurulation by varying the time point and pharmacological agent.
03:56
Inducing Traumatic Injury in a Brain Slice Using a Shock Tube Device
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2025
In this video, a shock tube device is used to generate a shockwave by rupturing a polymer diaphragm through controlled pressurization. The shockwave impacts brain slices contained in a secured bag, inducing traumatic injury to the brain slice.
05:23
A Modified Roller Tube Method for the Long-Term Culture of Rodent Brain Slices
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2025
This video demonstrates a modified roller tube method for the long-term culturing of brain slices from a rodent model. First, the brain slices are attached to coverslips. Then, flat-sided roller tubes with a hole on the flat side are taken, and the coverslips are positioned on the hole using adhesive discs. Finally, a nutrient medium and a carbon dioxide and air mixture are added to the tube, and the tubes are incubated with a rolling motion.
09:57
Dual Labeling of Neural Crest Cells and Blood Vessels Within Chicken Embryos Using ChickGFP Neural Tube Grafting and Carbocyanine Dye DiI Injection
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Cited by 11 •
2015
Here we report dual labeling of neural crest cells and blood vessels using chickGFP neural tube intraspecies grafting combined with intra-vascular DiI injection. This experimental technique allows us to simultaneously visualize and study development of the NCC-derived (enteric) nervous system and the vascular system, during organogenesis.
FAQs
Preserving the local architecture keeps neural progenitors, differentiating neurons, migrating cells, and growing axons in their tissue relationships rather than separating them into isolated populations. Those spatial relationships retain signaling interactions that help organize development, allowing investigators to connect a cellular behavior with the surrounding neural tube environment.
A neural tube slice occupies an intermediate experimental scale. Unlike isolated cellular assays, it preserves local tissue organization and signaling relationships. Unlike whole-embryo studies, it can be examined under controlled conditions and subjected to focused observation or manipulation. This combination helps link cellular mechanisms with larger-scale nervous-system organization.
Manipulating signaling pathways tests how developmental signals influence tissue organization and cell behavior within the preserved neural tube environment. Researchers can then examine effects on neural progenitor behavior, neuronal differentiation, migration, or axon growth. Because the tissue relationships remain intact, the results can reveal how signaling operates in a coordinated developmental setting.
The preparation supports concurrent study of neural progenitor behavior, neuronal differentiation, migration, and axon growth. Examining these processes within one organized tissue context helps researchers relate changes in one population or behavior to neighboring cells and signaling relationships. That integrated view is valuable for understanding how developing neural tissue becomes patterned and organized.
A typical analysis uses the preserved slice as the experimental tissue, applies controlled observation or signaling manipulation when needed, and then examines the preparation with microscopy or immunostaining. These approaches allow researchers to assess tissue organization, cell populations, developmental behaviors, and responses to altered signaling without relying solely on whole-embryo studies.
Microscopy reveals the organization and behavior of cells or growing axons within the slice, while immunostaining helps examine defined cell populations or developmental features. Experimental manipulation of signaling pathways adds a causal test to these observations. Used together, these methods connect visual tissue patterns with specific developmental processes.
Researchers use neural tube slices to investigate neural development, tissue patterning, circuit formation, and developmental disorders affecting the brain and spinal cord. The model is especially useful when experiments require preserved local relationships but also benefit from controlled conditions, direct imaging, immunostaining, or targeted manipulation of developmental signaling.
This preparation bridges cellular assays and whole-embryo studies. It preserves enough tissue organization to examine signaling relationships and coordinated developmental behaviors, while offering an experimentally accessible setting for observation and manipulation. Consequently, findings from the slice can contribute to broader interpretations of how neural tissue is patterned and how developmental abnormalities may arise.