Local Protein Synthesis
Local Protein Synthesis - Related Videos
05:19
An Immunofluorescence Technique for Viral Protein Localization in Infected Cells
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2025
This video demonstrates a method for monitoring infected cell protein 0, ICP0 trafficking in herpes simplex virus-1 infection. Post-de novo synthesis, ICP0 translocates to the nucleus, later moving to the cytoplasm during infection progression. Immunofluorescence microscopy reveals and analyzes the protein's subcellular localization, offering insights into its trafficking across infection phases.
09:14
Synthesis of Protein Bioconjugates via Cysteine-maleimide Chemistry
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Cited by 8 •
2016
This protocol details the important steps required for the bioconjugation of a cysteine containing protein to a maleimide, including reagent purification, reaction conditions, bioconjugate purification and bioconjugate characterization.
08:07
Identification of Plasmodesmal Localization Sequences in Proteins In Planta
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Cited by 6 •
2017
Plant intercellular connections, the plasmodesmata (Pd), play central roles in plant physiology and plant-virus interactions. Critical to Pd transport are sorting signals that direct proteins to Pd. However, our knowledge about these sequences is still in its infancy. We describe a strategy to identify Pd localization signals in Pd-targeted proteins.
13:13
Nano-fEM: Protein Localization Using Photo-activated Localization Microscopy and Electron Microscopy
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Cited by 14 •
2012
We describe a method to localize fluorescently tagged proteins in electron micrographs. Fluorescence is first localized using photo-activated localization microscopy on ultrathin sections. These images are then aligned to electron micrographs of the same section.
04:46
Synthesis of Nanodisc-Stabilized Membrane Protein Antigens Using a Cell-Free System
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2025
Source: Gilmore, S. F., et al. Cell-Free Scaled Production and Adjuvant Addition to a Recombinant Major Outer Membrane Protein from Chlamydia muridarum for Vaccine Development. J. Vis. Exp. (2022)This video demonstrates the cell-free synthesis of nanodisc-stabilized membrane protein antigens using a dual-compartment system. The device maintains optimal conditions for efficient protein synthesis, proper folding, and stable integration into nanodiscs.
FAQs
Transported messenger RNAs provide the transcripts that can be translated outside the cell body, while ribosomes carry out protein production near neuronal activity. Their presence in dendrites, axons, and synapses allows local signals to influence which proteins are produced at particular neuronal sites. This arrangement connects activity with protein availability in the compartment where cellular adaptation is needed.
Spatially restricted production lets neuronal activity regulate protein availability close to the affected synapse, dendrite, or axon. Instead of relying exclusively on production in the cell body, neurons can associate translation with the site receiving or generating a signal. This local arrangement helps explain how experience can produce compartment-specific changes that contribute to neuronal adaptation.
Synaptic stimulation can act as a signal that regulates translation of messenger RNAs located near synapses. By linking synaptic activity to protein production at the stimulated site, this mechanism supports changes in connection strength. Those changes include strengthening or weakening synaptic connections, processes central to synaptic plasticity and to the neural basis of learning and memory.
Local Protein Synthesis provides a way for synaptic activity to be associated with changes in protein production near individual connections. The resulting regulation supports synaptic plasticity, including both strengthening and weakening of synapses. Because these connection changes underlie learning and memory, local translation offers a mechanism for relating neuronal experience to lasting functional adaptation.
Axons contain sites where locally produced proteins can contribute to responses associated with growth and repair. Positioning protein production within axonal compartments allows neuronal processes to respond in the relevant part of the cell rather than depending exclusively on the cell body. This makes local translation important for understanding how neurons extend, maintain, and repair axonal structures.
Examining disruptions in local protein synthesis can help researchers connect altered protein production with impaired neuronal adaptation. Since local translation contributes to synaptic plasticity as well as axonal growth and repair, its disruption may affect how neurons modify connections or respond to structural damage. This research provides context for understanding neurological function and dysfunction.