Map Two Immunoreactivity
Map Two Immunoreactivity - Related Videos
05:59
Ultra-High-Speed Western Blot using Immunoreaction Enhancing Technology
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Cited by 4 •
2020
An ultra-high-speed western blotting technique is developed by improving the kinetics of antigen-antibody binding through cyclic draining and replenishing (CDR) technology in conjunction with an immunoreaction enhancing agent.
07:28
Visualizing the Calcitonin Gene-Related Peptide Immunoreactive Innervation of the Rat Cranial Dura Mater with Immunofluorescence and Neural Tracing
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Cited by 3 •
2021
Here we present a protocol to visualize spatial correlation of calcitonin gene-related peptide (CGRP)-immunoreactive nerve fibers and blood vessels in the cranial dura mater using immunofluorescence and fluorescent histochemistry with CGRP and phalloidin, respectively. In addition, the origin of these nerve fibers was retrograde traced with a fluorescent neural tracer.
09:33
An Acute Retinal Model for Evaluating Blood Retinal Barrier Breach and Potential Drugs for Treatment
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Cited by 3 •
2016
A low-cost, easy-to-use and powerful system is established to evaluate potential treatments that could ameliorate blood retinal barrier breach induced by histamine. Blood vessel leakage, Müller cell activation and the continuity of neuronal processes are utilized to assess the damage response and its reversal with a potential drug, lipoxin A4.
04:45
Quantification of Filamentous Actin Puncta in Rat Cortical Neurons
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2025
This video demonstrates the procedure for fluorescently staining rat cortical neurons for microscopy. It targets F-actin and dendritic proteins to analyze structural integrity and calculate F-actin density, which is crucial for studying neuronal function and health.
08:32
Sex Differences in Mouse Hippocampal Astrocytes after In-Vitro Ischemia
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Cited by 9 •
2016
Astrocytes are one of the most important key players in the central nervous system (CNS). Here, we are reporting a practical method of sexed hippocampal astrocyte culture protocol in order to study the mechanisms underlying the astrocyte function in male and female neonate pups after in-vitro ischemia.
FAQs
The primary antibody recognizes MAP2 epitopes, which are specific regions of the protein accessible for antibody binding. A labeled secondary antibody then binds the primary antibody and produces a detectable signal. This indirect detection step amplifies the visual readout, allowing investigators to map where MAP2 is concentrated within fixed tissue or cultured cells.
MAP2 is particularly enriched in dendrites, so its staining pattern provides a molecular marker for comparing neuronal compartments. When the signal is examined alongside cellular structure, researchers can identify dendritic regions and contrast them with axonal regions. This distinction supports analyses of neuronal polarity, compartment organization, and changes in dendritic architecture.
Variation in staining distribution or intensity can provide information about neuronal morphology, maturation, and structural responses to experimental conditions. Researchers may examine changes in dendritic architecture or the organization of neuronal processes after injury or treatment. The resulting patterns are interpreted as structural measurements rather than as a complete measure of neuronal function.
A typical workflow begins with tissue or cultured cells, followed by fixation to preserve cellular structure. The sample is exposed to a primary antibody directed against MAP2 and then to a labeled secondary antibody. Visualization of the resulting signal reveals MAP2 distribution, which can subsequently be assessed across cells, processes, or experimental groups.
The approach can be performed on fixed tissue or cultured cells. Its essential detection components are a primary antibody that binds MAP2 epitopes and a labeled secondary antibody that makes the bound primary antibody visible. These components allow the same general strategy to examine MAP2 organization in both experimental preparations.
Researchers may select this approach when they need to identify neurons, examine dendritic structure, or evaluate neuronal maturation. It is also useful for assessing structural changes associated with injury, experimental treatment, or neurodegenerative processes. Because the signal maps a neuronal cytoskeletal protein, the method connects molecular labeling with observable cellular morphology.
Quantifying staining patterns can support comparisons of MAP2 distribution and dendritic architecture between samples or experimental conditions. These measurements may help characterize neuronal development, maturation, injury-related structural changes, or treatment-associated effects. Interpretation depends on the observed staining pattern and its relationship to the morphology of the labeled neuronal cells.