Mouse Hindbrain Culture
Mouse Hindbrain Culture - Related Videos
10:57
Mouse Hindbrain Ex Vivo Culture to Study Facial Branchiomotor Neuron Migration
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Cited by 4 •
2014
Embryonic neurons are born in the ventricular zone of the neural tube, but migrate to reach appropriate targets. Facial branchiomotor (FBM) neurons are a useful model to study neuronal migration. This protocol describes the wholemount ex vivo culture of mouse embryo hindbrains to investigate mechanisms that regulate FBM migration.
11:39
The Mouse Hindbrain As a Model for Studying Embryonic Neurogenesis
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Cited by 2 •
2018
This article demonstrates how the mouse embryonic hindbrain can be used as a model for studying developmental neurogenesis in both whole organ and tissue section preparations.
09:17
Labeling and Imaging Cells in the Zebrafish Hindbrain
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Cited by 7 •
2010
Key to understanding the morphogenetic processes that shape the early embryo is the ability to image cells at high resolution. We describe here a technique for labeling single cells or small clusters of cells in whole zebrafish embryos with membrane-targeted Green Fluorescent Protein.
07:57
Isolation and Culture of Oculomotor, Trochlear, and Spinal Motor Neurons from a Mouse Embryo
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2025
The video demonstrates the isolation of oculomotor, trochlear, and spinal motor neurons from a transgenic mouse embryo. The embryo expresses motor neurons labeled with a green fluorescent protein (GFP) for easy visualization and identification. The motor neurons are surgically isolated under a fluorescence microscope and maintained in a culture medium.
08:47
Culturing Mouse Cardiac Valves in the Miniature Tissue Culture System
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Cited by 10 •
2015
Here, we present an ex vivo flow model in which murine cardiac valves can be cultured allowing the study of the biology of the valve.
FAQs
The preparation determines which biological relationships remain available for study. Tissue cultures preserve a more organized local environment, whereas dissociated cultures separate cells so researchers can examine individual neurons, glia, or neural progenitors under controlled conditions. This distinction helps investigators match the model to questions about circuit development, axon growth, differentiation, or cell signaling.
Researchers can follow how hindbrain neurons and neural progenitors change over time, including neuronal differentiation, axon growth, and signaling between cells. Glial responses can also be examined within the same general model. These readouts provide a way to connect cellular behavior with processes that contribute to developing neural circuits and maintaining nervous-system function.
Controlled conditions allow investigators to examine cellular responses outside the organism while focusing on selected experimental influences. By observing neurons, glia, or progenitors in a defined in vitro setting, researchers can study changes in differentiation, signaling, or axon growth with fewer biological factors operating simultaneously. This supports clearer analysis of cellular mechanisms relevant to neuroscience.
A general workflow begins by isolating hindbrain tissue, followed by either maintaining the tissue as a culture or dissociating it into cells. The preparation is then sustained under controlled culture conditions so that neurons, glia, or neural progenitors remain available for observation. Researchers can subsequently examine development, signaling, axon growth, or responses to experimental treatments.
This model is useful when a study requires direct observation of hindbrain cells or tissue rather than measurements only from an intact organism. It can support investigations of neuronal differentiation, circuit development, axon growth, cell signaling, neural injury, neurodevelopmental disorders, and responses to experimental treatments. The appropriate application depends on the cellular process and outcome being examined.
Cultured hindbrain preparations can reveal how cells respond to conditions associated with neurodevelopmental disorders or neural injury, and how they react to experimental treatments. Such observations do not replace organism-level research, but they help identify cellular mechanisms and responses that may explain broader nervous-system changes. The model therefore links controlled cellular experiments with disease-focused neuroscience questions.