The Mouse Hindbrain As a Model for Studying Embryonic Neurogenesis

Journal

Neuroscience

JoVE Journal
Neuroscience

This content is Free Access.

JoVE Journal Neuroscience

The Mouse Hindbrain As a Model for Studying Embryonic Neurogenesis

DOI:

10.3791/56793-v

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11:39 min

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January 29, 2018

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Mathew Tata, Christiana Ruhrberg

¹UCL Institute of Ophthalmology

Chapters

00:05Title
00:40Dissection of Hindbrains from e9.5–e13.5 Mouse Embryos
03:34Wholemount Immunofluorescence
05:59Vibratome Sectioning and Floating Section Immunofluorescence
07:12Cryostat Sectioning and Cryosection Immunofluorescence Labeling and Image Acquisition
08:58Results: Wholemount and Tissue Section Analysis of Hindbrain Neurogenesis
10:20Conclusion

Summary

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This article demonstrates how the mouse embryonic hindbrain can be used as a model for studying developmental neurogenesis in both whole organ and tissue section preparations.

The Mouse Hindbrain As a Model for Studying Embryonic Neurogenesis

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