Nerve Suturing

Nerve suturing is a microsurgical technique that reconnects the ends of a severed peripheral nerve, restoring continuity needed for functional recovery. Surgeons carefully align the nerve ends and their fascicles under magnification, then place fine sutures through the surrounding epineurium or individual fascicular layers to stabilize the repair; regenerating axons can subsequently extend across the injury with support from Schwann cells. In neuroscience and reconstructive surgery, nerve suturing provides a model for studying axonal regeneration and guides treatment of traumatic nerve injuries. Outcomes depend on accurate alignment, injury severity, repair timing, and the distance regenerating axons must traverse.

Nerve Suturing - Related Videos

Research

JoVE Journal - Medicine
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Iris Fixation via External Pentagram Suturing

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2022

The present protocol describes how a 10-0 polypropylene suture crosses the anterior surface of the iris, forming a pentagram to prevent both iris and pupil from moving toward the cornea. This can be combined with subsequent keratoplasty to cure bullous keratopathy with extensive anterior synechia of the iris.

Research

JoVE EoE - Large Animal Models

Q Suture Technique: A Suturing Technique to Reduce Gap Formation and Increase Tensile Strength in Tendon Repair

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2025

In this video, we demonstrate suturing techniques — core suture and Q suture — to repair the foot flexor tendon in an ex vivo porcine model. Q sutures enhance the strength of the core suture in tendon repair and decrease gapping at the tendon repair site.

Construction of a Composite Regenerative Peripheral Nerve Interface in a Rat Model of Nerve Injury

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2025

Source: Svientek, S. R., et al. Fabrication of the Composite Regenerative Peripheral Nerve Interface (C-RPNI) in the Adult Rat. J. Vis. Exp. (2020).This video demonstrates the surgical construction of a composite regenerative peripheral nerve interface (C-RPNI) in a rat model of peripheral nerve injury. The procedure involves attaching a transected peroneal nerve to a muscle graft and overlaying it with a dermal skin graft to promote nerve regeneration. Over time, motor and sensory neurons...

Education

JoVE Core - Anatomy and Physiology

Sutures of the Skull

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2025

The human skull is composed of several bones that come together to protect the brain and support the structures of the face. The junctions where these bones meet are called sutures. Sutures are immobile joints between adjacent bones of the skull. The narrow gap between the bones is filled with dense, fibrous connective tissue that unites the bones. The long sutures located between the skull bones are not straight but instead follow irregular, tightly twisting paths. These twisting lines tightly...

Minimally-invasive Technique for Injection into Rat Optic Nerve

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Cited by 4 •

2015

Direct injection into the rat optic nerve is useful for regenerative research. We demonstrate a minimally-invasive technique for direct injection into a rat optic nerve that does not involve opening the skull. Using this method, surgical complications are minimized and recovery is more rapid.

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