Neuronal Surface Antigens
Neuronal Surface Antigens - Related Videos
13:05
Flow Cytometry Protocols for Surface and Intracellular Antigen Analyses of Neural Cell Types
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Cited by 39 •
2014
We provide a detailed description of a protocol for flow cytometric analysis of surface antigens and/or intracellular antigens in neural cell types. Critical aspects of experimental planning, step-by-step methodological procedures, and fundamental principles of flow cytometry are explained in order to enable neurobiologists to exploit this powerful technology.
05:04
Viral Antigen Microarray Assay to Detect Antibody Isotypes in Serum Against Viral Antigen
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2025
In this video, we demonstrate the process for detecting antibody isotypes in multiple human serum samples using a microarray slide printed with antigenic subtypes of influenza virus strains.
06:57
Development of an Antigen-driven Colitis Model to Study Presentation of Antigens by Antigen Presenting Cells to T Cells
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Cited by 9 •
2016
In this antigen-driven colitis model, OT-II CD4+ T cells expressing a red fluorescent protein were adoptively transferred into RAG-/- mice that express a green fluorescent protein in mononuclear phagocytes (MPs). The hosts were challenged with Escherichia coli (E.coli) expressing the ovalbumin protein (OVA) fused to a cyan fluorescent protein (CFP).
03:47
Crosslinking Neuronal Surface Proteins in the Mouse Brain using a Chemical Crosslinking Assay
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2025
This video demonstrates the crosslinking of neuronal surface proteins using Bis-sulfosuccinimidyl suberate or BS3. Isolated and chilled mouse brain is sectioned coronally. The desired region is extracted from the sections, minced, and treated with BS3. BS3 crosslinks surface proteins like GABA receptors, leaving internal proteins unaltered. The reaction is quenched with glycine before processing the samples for further analysis.
11:56
Differential Labeling of Cell-surface and Internalized Proteins after Antibody Feeding of Live Cultured Neurons
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Cited by 24 •
2014
We describe a method to label protein on the surface of living neurons using a specific polyclonal antibody to extracellular epitopes. Protein bound by the antibody on the cell surface and subsequently internalized via endocytosis can be distinguished from protein remaining on, or trafficked to, the surface during the incubation.
FAQs
These antigen classes reflect different aspects of neuronal biology. Receptors interact with extracellular signals, ion channels contribute to neuronal responses, and cell-adhesion molecules support connectivity between cells. Examining their combined presence therefore provides more information than treating a single antigen as a complete identifier, helping researchers relate cell identity to communication, connectivity, and functional state.
Surface-antigen patterns can reflect how neurons interact with signals outside the cell. Because receptors and other membrane molecules participate in communication and responses, changes in their display may correspond to altered functional states. Measuring these profiles allows researchers to examine neuronal populations not only by identity, but also by differences in their signaling-related condition.
A profile combines multiple detectable molecules rather than relying on one feature alone. Differences in receptors, ion channels, or adhesion molecules can help identify and distinguish neuronal populations while also indicating functional characteristics. This supports cell classification and gives researchers a way to connect population differences with neural connectivity, communication, or responses.
Disease-associated changes may appear as altered surface-antigen patterns, providing measurable evidence that neuronal properties have changed. These molecules can also participate in interactions between neurons and immune-related signals, making their profiles relevant to neuroimmune studies. Such findings may help identify disease-associated features and highlight surface molecules for investigation as potential therapeutic targets.
Researchers can detect these molecules with antibodies, immunostaining, flow cytometry, or other labeling methods. Antibody-based approaches identify selected surface molecules, while labeling-based analyses make their distribution or presence measurable. The chosen method enables researchers to examine neuronal populations and compare antigen patterns in relation to development, function, connectivity, or disease-associated change.
Immunostaining and related labeling approaches can show where selected surface antigens occur among neurons. Their patterns help map neuronal populations and examine how cells differ in their antigen profiles. In neuroscience, this information supports cell classification and neural circuit analysis by linking molecular features on neurons with their distribution and connectivity.
Flow cytometry is useful when researchers need to analyze labeled surface-antigen patterns across neuronal populations. By detecting antibody- or label-associated signals, it supports comparison of cells according to their molecular profiles. This makes the method relevant to population classification and to studies of functional or disease-associated differences identified through surface-antigen changes.