Peptide-sirna Complex
Peptide-sirna Complex - Related Videos
02:57
Targeted siRNA Delivery to Neurons for Prion Protein Silencing Using Liposomal Complexes
0 Views •
2025
Source: Bender, H. R., et.al. Delivery of Therapeutic siRNA to the CNS Using Cationic and Anionic Liposomes. J. Vis. Exp. (2016)This video demonstrates the targeted delivery of prion protein (PrP) siRNA to neurons using liposomal siRNA-peptide complexes (LSPCs). Cationic liposomes electrostatically bind PrP-targeting siRNA, which is further stabilized by the RVG-9r peptide for receptor-mediated transport across the blood-brain barrier. Once inside the brain, LSPCs undergo endocytosis in...
02:30
siRNA - Small Interfering RNAs
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2020
Small interfering RNAs, or siRNAs, are short regulatory RNA molecules that can silence genes post-transcriptionally, as well as the transcriptional level in some cases. siRNAs are important for protecting cells against viral infections and silencing transposable genetic elements. In the cytoplasm, siRNA is processed from a double-stranded RNA, which comes from either endogenous DNA transcription or exogenous sources like a virus. This double-stranded RNA is then cleaved by the ATP-dependent...
04:50
Phosphopeptide Enrichment: An Antibody-based Immunoprecipitation Technique to Separate Specific Phosphorylated Peptides from Complex Peptide Mixtures
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2023
In this video, we demonstrate antibody-based immunoprecipitation of phosphopeptides from a peptide mixture. The enriched phosphopeptides are vacuum concentrated and stored for downstream analysis.
12:05
Production of Disulfide-stabilized Transmembrane Peptide Complexes for Structural Studies
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Cited by 7 •
2013
Biophysical and biochemical studies of interactions among membrane-embedded protein domains face many technical challenges, the first of which is obtaining appropriate study material. This article describes a protocol for producing and purifying disulfide-stabilized transmembrane peptide complexes that are suitable for structural analysis by solution nuclear magnetic resonance (NMR) and other analytical applications.
04:05
Intranasal Delivery of siRNA to the Mouse Brain Using a Positioning Device
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2025
Source: Ullah, I., et.al. A Positioning Device for the Placement of Mice During Intranasal siRNA Delivery to the Central Nervous System. J. Vis. Exp. (2019)This video demonstrates a precise intranasal administration technique for delivering neuron-targeting peptide-siRNA complexes to the mouse brain using a specialized positioning device. Anesthetized mice are secured in a head-down-and-forward position to optimize siRNA inhalation while preventing lung drainage. The targeting peptide...
FAQs
Binding is driven mainly by electrostatic attraction: positively charged peptide residues associate with negatively charged phosphate groups along siRNA. This interaction condenses the nucleic acid into a more compact assembly, which can help protect siRNA and support its movement toward cells. The peptide's charge-related interaction is therefore central to complex formation and delivery.
Intracellular release makes the siRNA available after the complex has entered a cell. Once released, the siRNA can guide sequence-specific degradation of complementary messenger RNA. This step connects physical delivery with functional gene regulation, because a complex that protects or transports siRNA but does not release it cannot effectively expose the silencing molecule to its target messenger RNA.
The principal interaction depends on the charge relationship between the two components. Positively charged residues on the peptide attract the negatively charged phosphate backbone of siRNA, allowing the nucleic acid to become condensed. This charge-based association distinguishes complex formation from the later biological event, in which siRNA recognizes complementary messenger RNA for sequence-specific degradation.
A conceptual workflow begins with associating the positively charged peptide with siRNA, allowing electrostatic binding and condensation to produce the complex. The assembly is then investigated for cellular uptake, followed by intracellular siRNA release and degradation of complementary messenger RNA. In a study, these stages connect complex formation with the intended gene-silencing outcome.
In neuroscience, these complexes provide a strategy for studying gene regulation in pathways associated with neuronal signaling, neurodegeneration, inflammation, and cancer. Silencing selected genes can help researchers examine how those genes contribute to nervous-system processes or disease-related mechanisms. Their ability to support siRNA stability and delivery makes them relevant to both molecular studies and therapeutic development.
The principal outcome is sequence-specific reduction of complementary messenger RNA after intracellular release of siRNA. This enables researchers to investigate the consequences of lowering expression of genes linked to neuronal signaling, neurodegeneration, inflammation, or cancer. Results can therefore connect a defined gene-silencing event with broader questions about nervous-system function and disease mechanisms.